PXD055780-1

PXD055780 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MSIght: A Modular Platform for Improved Confidence in Global, Untargeted Mass Spectrometry Imaging Annotation
Description	Mass spectrometry imaging (MSI) has gained popularity in large-scale clinical analyses due to its high sensitivity, specificity and throughput. However, global profiling experiments are often still restricted to LC-MS/MS analyses that lack spatial localization due to low throughput methods for on-tissue peptide confirmation. Parallel LC-MS/MS confirmation of extracted peptides is often utilized with MALDI-MSI; however, integration and analysis of the two data sets once again presents a bottleneck in throughput. Further, the incorporation of histological stains is critical for mapping identified peptides of interest back to relevant tissue regions, yet many software generated for this are not readily accessible to those lacking coding capabilities. Here, we present a novel platform termed MSIght, which automates the integration of these multiple modalities into an accessible and modular platform. Histological stains of tissue sections are co-registered to their respective MSI data sets to improve spatial localization and resolution of identified peptides. LC-MS/MS data of extracted peptides is used to confirm putative MSI identifications and generate confident MS images in an untargeted manner. This platform enables large-scale clinical cohorts to utilize MSI for global proteomic profiling to uncover novel biomarkers in a spatial manner, thus widely expanding the utility of MSI in clinical discovery.
HostingRepository	MassIVE
AnnounceDate	2024-12-18
AnnouncementXML	Submission_2024-12-18_14:25:42.929.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauren Fields
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	rapifleX; Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-11 06:42:40	ID requested
⏵ 1	2024-12-18 14:25:43	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: MALDI, multimodal imaging, mass spectrometry imaging, histology

submitter keyword: MALDI, multimodal imaging, mass spectrometry imaging, histology

Contact List

Lingjun Li
contact affiliation	University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Lauren Fields
contact affiliation	UW Madison
contact email	lawashburn@wisc.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095830/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095830/