PXD055774-1

PXD055774 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry was used to identify proteins that bind to S3B
Description	H1975 cells were treated with LS1-Biotin-S3B (2 μg/mL) or Biotin for 72 hours. After treatment, the cells were harvested and lysed in a buffer supplemented with a protease inhibitor. The cell lysate (600 μL) was incubated with 25 μL of streptavidin magnetic beads (MCE#HY-K0208) at 4°C for 4-6 hours with rotation. Following incubation, the beads were washed five times with PBST washing buffer, with excess buffer carefully removed after the final wash. The proteins pulled down from the beads were separated by SDS-PAGE and then silver-stained. Differential gel bands were excised, and the proteins on the gel bands were analyzed by mass spectrometry.
HostingRepository	iProX
AnnounceDate	2024-09-12
AnnouncementXML	Submission_2025-07-30_19:43:52.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuejiao Liang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Plus MALDI TOF/TOF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-11 00:52:01	ID requested
⏵ 1	2025-07-30 19:43:53	announced

Dataset with its publication pending

submitter keyword: Act1，SHP2 stability, NSCLC

Qiong Luo
contact affiliation	Nanjing University
contact email	luoyiqing8@163.com
lab head
Xuejiao Liang
contact affiliation	Nanjing University
contact email	xuejiao_liang@126.com
dataset submitter

iProX dataset URI