PXD055763-1

PXD055763 is an original dataset announced via ProteomeXchange.

Title	VCP enhances autophagy-related osteosarcoma progression by recruiting USP2 to inhibit ubiquitination and degradation of FASN
Description	The mass spectrometry data were acquired using a Q Exactive HF mass spectrometer coupled with an UltiMate 3000 RSLCnano liquid chromatography system. Peptide samples were dissolved in a loading buffer, introduced via an autosampler, and separated on an analytical column (75 μm × 25 cm, C18, 1.9 μm, 120 Å). An analytical gradient was established using two mobile phases (mobile phase A: 0.1% formic acid, 3% DMSO; mobile phase B: 0.1% formic acid, 3% DMSO, 80% ACN). The flow rate of the liquid phase was set to 300 nL/min. Data were acquired in DDA mode, with each scan cycle including one MS full scan (R = 60 K, AGC = 3e6, max IT = 25 ms, scan range = 350–1500 m/z), followed by 20 MS/MS scans (R = 15 K, AGC = 1e5, max IT = 50 ms). The HCD collision energy was set to 27. The quadrupole isolation window was set to 1.4 Da, and the dynamic exclusion time for ion repeat acquisition was set to 24 s.
HostingRepository	iProX
AnnounceDate	2024-09-10
AnnouncementXML	Submission_2024-12-22_19:41:13.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shijiang Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-10 00:22:24	ID requested
⏵ 1	2024-12-22 19:41:14	announced

Wang S, Nie J, Jiang H, Li A, Zhong N, Tong W, Yao G, Jiang A, Xie X, Zhong Y, Shu Z, Liu J, Yang F, Liu Z, VCP enhances autophagy-related osteosarcoma progression by recruiting USP2 to inhibit ubiquitination and degradation of FASN. Cell Death Dis, 15(11):788(2024) [pubmed]

submitter keyword: mass spectrometry

Zhili Liu
contact affiliation	The First Affiliated Hospital of Nanchang University
contact email	zhili-liu@ncu.edu.cn
lab head
Shijiang Wang
contact affiliation	The First Affiliated Hospital of Nanchang University
contact email	wangshijiang2022@163.com
dataset submitter

iProX dataset URI