PXD055734-1

PXD055734 is an original dataset announced via ProteomeXchange.

Title	Optimizing Small and Large Brain-Derived Extracellular Vesicle Purification Reveals Critical Pathways in Alzheimer's Disease Patients
Description	At this day, Alzheimer’s disease is the most prominent form of dementia worldwide. It is characterized by tau lesions that spread throughout the brain in a spatio-temporal manner. This has led to the prion-like propagation hypothesis implicating a transfer of pathological tau seeds from cell-to-cell. Extracellular vesicles have been described as one of the mechanisms contributing to this intercellular transfer of tau seeds. Thus far, we and others demonstrated that human EVs from the brain-derived fluid (BD-EVs) of AD patients contain the capacity to induce tau seeding in vitro and in vivo. Based on these findings and the rich diversity of BD-EVs, we studied BD-EVs sub-populations in AD patients. Here, enriched-small and enriched-large EVs were isolated from two distinct enzymatic brain dissociation protocols. Proteomic analysis then aimed to define the optimal brain dissociation enzyme resulting in well-preserved EVs. We demonstrated that collagenase enzymatic brain dissociation not only results in a high EVs protein yield but also allowed us to detect more transmembrane proteins for both enriched-large and enriched-small EVs in comparison to papain dissociation. Proteomic content analysis of collagenase-derived AD-EVs revealed the increased presence of integrin-mediated synaptic signalling, brain-immunity and GWAS-associated proteins. Although, enriched-large EVs contain more GWAS-associated proteins then enriched-small EVs. This enriched small and large EV sub-population characterization drives comprehension of AD associated pathophysiologic mechanisms potentially mediated through EVs.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:46:05.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soulaimane Aboulouard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-09 01:34:40	ID requested
⏵ 1	2025-12-14 16:46:06	announced

10.1186/s40035-025-00519-z;

Oosterlynck M, Leroux E, Namasivayam B, Bouillet T, Caillierez R, Loyens A, Mazur D, Perbet R, Lefebvre C, Aboulouard S, Maurage CA, Accart B, Bu, é, e L, Colin M, Stratification of brain-derived extracellular vesicles of Alzheimer's disease patients indicates a unique proteomic content and a higher seeding capacity of small extracellular vesicles. Transl Neurodegener, 14(1):63(2025) [pubmed]

submitter keyword: CLU, collagenase brain dissociation, FERMT2, extracellular vesicles, GWAS, proteomic profiling,Alzheimer's disease

Michel Salzet
contact affiliation	PRISM - U1192 Universite de Lille FRANCE
contact email	michel.salzet@univ-lille.fr
lab head
Soulaimane Aboulouard
contact affiliation	PRISM University of Lille, INSERM U1192
contact email	soulaimane.aboulouard@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD055734

PRIDE project URI

• PRIDE
  1. PXD055734
     1. Label: PRIDE project
     2. Name: Optimizing Small and Large Brain-Derived Extracellular Vesicle Purification Reveals Critical Pathways in Alzheimer's Disease Patients