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PXD055614-1

PXD055614 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteins of sheep abomasum that interact with Hc-SPI-I8
DescriptionFreshly collected abomasum tissue of the naturally infected sheep was ground, filtered, and centrifuged at 3000 g at 4 °C for cell collection. The collected cells were lysed in NP-40 lysis buffer (Thermo Fisher Scientific) for 0.5 h with shaking, then centrifuged at 12000 g at 4 °C for supernatant collection. Coding sequence of Hc-spi-i8 (HCON_00067680) was amplified and inserted into pcDNA3.1(+) vector to produce FLAG-tagged Hc-SPI-I8 in HEK 293T cells. The recombinant Hc-SPI-I8-FLAG protein was isolated from lysed cells using anti-FLAG-conjugated magnetic beads (Bimake, Shanghai, China), and used to isolate and concentrate interactive proteins from the ovine tissue lysis. The isolated proteins were boiled, separated by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), from which proteins within a range of molecular weights from 30 to 45 kDa were subjected to digestion with trypsin (Promega), desalination with a sep-Pak C18 (Waters) and LC-MS/MS analysis with a Q-Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific). Peptides were identified and annotated using Proteome Discoverer v.2.1.
HostingRepositoryiProX
AnnounceDate2024-09-06
AnnouncementXMLSubmission_2024-09-05_23:45:35.965.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFei Wu
SpeciesList scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-05 23:44:55ID requested
12024-09-05 23:45:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Haemonchus contortus, IP-MS, host-parasite interplay, protein-protein interaction
Contact List
Guangxu Ma
contact affiliationZhejiang University
contact emailgxma1@zju.edu.cn
lab head
Fei Wu
contact affiliationZhejiang University
contact emailwufei0214@zju.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI