PXD055597-1

PXD055597 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisonsProteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisons
Description	Extracellular vesicles (EVs) are key in intercellular communication, carrying biomolecules like nucleic acids, lipids, and proteins. This study investigated postprandial characteristics and proteomic profiles of plasma-derived large extracellular vesicles (lEVs) in healthy individuals. Twelve participants fasted overnight before baseline assessments. After consuming a controlled isocaloric meal, EVs were isolated for proteomic and flow cytometric analysis. Plasma triacylglyceride (TAG) levels confirmed fasting completion, while protein concentrations in plasma and EVs were monitored for postprandial stability. Proteomic analysis identified upregulated proteins related to transport mechanisms and epithelial/endothelial functions postprandially, indicating potential roles in physiological responses to nutritional intake. Enrichment analyses revealed vesicle-related pathways and immune system processes. Flow cytometry showed increased expression of CD324 on CD9+CD63+CD81+ EVs postprandially, suggesting an epithelial origin. These findings offer insights into postprandial lEV dynamics and their physiological significance, highlighting the need for stringent fasting guidelines in EV studies to account for postprandial effects on EV composition and function.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:18:25.369.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lothar Jaensch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-05 12:56:24	ID requested
⏵ 1	2025-05-07 04:18:26	announced

10.1038/s41598-024-74228-4;

Garza AP, Wider-Ebersp, ä, cher E, Morton L, van Ham M, P, á, llinger É, Buz, á, s EI, J, ä, nsch L, Dunay IR, Proteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisons. Sci Rep, 14(1):23032(2024) [pubmed]

submitter keyword: proteomics, fasting,Postprandial, extracellular vesicles, plasma, liquid biopsy

Lothar Jaensch
contact affiliation	Cellular Proteome Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
contact email	Lothar.Jaensch@Helmholtz-HZI.de
lab head
Lothar Jaensch
contact affiliation	HZI
contact email	Lothar.Jaensch@Helmholtz-HZI.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055597

PRIDE project URI

• PRIDE
  1. PXD055597
     1. Label: PRIDE project
     2. Name: Proteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisonsProteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisons