PXD055562 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Serum proteomic analysis of circulating IgA1-containing immune complexes in patients with IgA nephropathy |
| Description | IgA nephropathy (IgAN) is an autoimmune disease that involves galactose-deficient IgA1 (Gal-deficient IgA1) recognized by autoantibodies to form circulating immune complexes (IgA1-IC). Some of these complexes deposit in the glomeruli and induce kidney injury. Gal-deficient IgA1 alone is not sufficient to induce kidney damage. Additional proteins are required to form nephritogenic complexes. In this study, we isolated different molecular forms of serum IgA1, including IgA1-IC, monomeric (mIgA1), and polymeric (pIgA1) by SEC from patients with IgAN and healthy control donors. These three chromatographic fractions of molecular forms of IgA1 were analyzed by LC-MS. An analysis of the proteomes of the IgA1 molecular forms yielded several serum proteins enriched in the IgA1-IC. These proteins were also enriched in patients with IgAN when compared to healthy controls. The ability to characterize the proteome of IgA1-IC provides critical information relevant to IgAN pathogenesis and the nephritogenic characteristics of the ICs. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-03 |
| AnnouncementXML | Submission_2025-11-03_02:13:01.575.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055562 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mary Cunningham |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-09-04 13:26:48 | ID requested | |
| ⏵ 1 | 2025-11-03 02:13:03 | announced | |
Publication List
| 10.1016/j.ekir.2025.07.005; |
| Cunningham MA, Hargett AA, Holloway AK, Hall SD, Craine EP, Maillard N, Rizk DV, Julian BA, Novak J, Renfrow MB, Proteomic Analysis of Circulating IgA1-Containing Immune Complexes in Patients with IgA Nephropathy. Kidney Int Rep, 10(10):3611-3623(2025) [pubmed] |
| 10.6019/PXD055562; |
Keyword List
| submitter keyword: proteomics, LC-MS, complement, orbitrap, raw, immune complexes,serum, IgA nephropathy, glomerular disease |
Contact List
| Matthew B. Renfrow |
|---|
| contact affiliation | Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham |
| contact email | renfrow@uab.edu |
| lab head | |
| Mary Cunningham |
|---|
| contact affiliation | University of Alabama at Birmingham |
| contact email | bunten@uab.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD055562 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055562
- Label: PRIDE project
- Name: Serum proteomic analysis of circulating IgA1-containing immune complexes in patients with IgA nephropathy
