PXD055536-1

PXD055536 is an original dataset announced via ProteomeXchange.

Title	The impact of exercise on skeletal muscle proteome of prediabetic subjects analyzed with data independent mass spectrometry.
Description	Skeletal muscle is a primary tissue responsible for both the exercise capacity and insulin-induced glucose uptake. Exercise-evoked proteomic alternations in muscle of subjects with prediabetes are of great importance for the elucidation of the connections between insulin resistance and physical exercise. To envision best aviable approach, we compared various ion-chromatogram libraries assembled with the use of off-line high-pH fractionation (HpH), gas-phase fractionation (GPF) and library-less DirectDIATM approach in LC/MS/HRMS DIA proteomic analysis of muscle from normoglycemic (NGT) and prediabetic (IGT) subjects after 3 months of supervised, mixed-mode exercise. Although HpH approach to library generation resulted in best proteome coverage, quantitative and qualitative analysis of HeLa standards, mixed samples and whole study set revealed, that GPF-fractionated hybrid DDA/DIA-acquired libraries yielded best overall balance between the speed of preparation, data collection, data analysis and identification in real-life clinical samples.
HostingRepository	PRIDE
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-22_08:28:21.852.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Piotr Zabielski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-04 03:23:17	ID requested
⏵ 1	2025-07-22 08:28:22	announced

Dataset with its publication pending

submitter keyword: gas-phase fractionation, GPF, DIA, prediabetes, exercise, Homo sapiens,skeletal muscle, high-pH fractionation, HpH

Piotr Zabielski
contact affiliation	Department of Medical Biology, Medical University of Bialystok, Bialystok, Poland
contact email	piotr.zabielski@umb.edu.pl
lab head
Piotr Zabielski
contact affiliation	Medical University of Bialystok, Department of Medical Biology
contact email	piotr.zabielski@umb.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055536
     1. Label: PRIDE project
     2. Name: The impact of exercise on skeletal muscle proteome of prediabetic subjects analyzed with data independent mass spectrometry.