PXD055516 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of isolated nerve terminals from NaV1.9 knockout mice reveals pathways relevant for pain signaling |
| Description | Chronic pain substantially affects the mental and physical well-being of patients and magnifies the socio-economic burden on the healthcare system. It is important to understand the molecular mechanisms underlying chronic pain to effectively target it. To investigate peripheral mechanisms relevant to pain signaling, we isolated nerve terminals from mouse footpads. The isolated peripheral terminals contain both pre- and post-synaptic proteins and are deficient in keratin and histone in both mice and humans. We detected the protein translational machinery and mitochondria in nerve terminals and observed that they were capable of endocytosis. An unbiased proteomic analysis of nerve terminals from footpads of NaV1.9 knockout mice shows dysregulation of the p38 mitogen-activated protein kinase (MAPK) and extracellular regulated kinase 1/2 (ERK1/2) pathways, and of protein components involved in translation and energy metabolism. Our study thus reveals peripheral signaling mechanisms implicated in pain perception. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-24 |
| AnnouncementXML | Submission_2025-11-23_17:43:07.950.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mario Oroshi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-09-03 06:08:30 | ID requested | |
| ⏵ 1 | 2025-11-23 17:43:08 | announced | |
Publication List
| 10.1097/j.pain.0000000000003743; |
| Rawat A, Vu DT, Erbacher C, Stigloher C, Ü, ç, eyler N, Metousis A, Mann M, Briese M, Sendtner M, Proteomic analysis of isolated nerve terminals from Na V 1.9 knockout mice reveals pathways relevant for pain perception. Pain, 166(12):e770-e787(2025) [pubmed] |
Keyword List
| submitter keyword: proteomics |
Contact List
| Matthias Mann |
|---|
| contact affiliation | Max Planck Institute of Biochemistry - Department Proteomics and Signal Transduction |
| contact email | mmann@biochem.mpg.de |
| lab head | |
| Mario Oroshi |
|---|
| contact affiliation | Proteomics |
| contact email | oroshi@biochem.mpg.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055516
- Label: PRIDE project
- Name: Proteomic analysis of isolated nerve terminals from NaV1.9 knockout mice reveals pathways relevant for pain signaling
