PXD055498-1

PXD055498 is an original dataset announced via ProteomeXchange.

Title	The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3
Description	The subcortical maternal complex (SCMC) is essential for the maintenance of female fertility in mammals. Most pathogenic mutations in SCMC-encoding genes result in female reproductive failure characterized by early embryonic arrest. Assembled in oocytes, the SCMC maintains the normal cleavage of early embryos, but the mechanism regulating this function remains unclear. Here, we report that 14-3-3, a multifunctional protein, is a component of the SCMC. By resolving the cryoelectron microscopy structure of the 14-3-3-containing SCMC, we revealed that specific phosphorylation sites in TLE6 contribute to the recruitment of 14-3-3, which is indispensable for the role of the SCMC in maintaining the cleavage of mouse early embryos. Mechanistically, during the maternal-to-embryo transition, the SCMC stabilizes the 14-3-3 protein, and an acute reduction in 14-3-3 protein expression in zygotes impairs their further development to blastocysts. The SCMC containing 14-3-3 contributes to the proper control of CDC25B, thus ensuring the activation of the maturation-promoting factor and mitotic entry in mouse zygotes. Notably, we proved that the recruitment of 14-3-3 to the SCMC and the molecular link between the SCMC and CDC25B are conserved in human oocytes/embryos. This study reveals the molecular mechanism through which the SCMC regulates the cell cycle in early embryos and represents a breakthrough in fully elucidating the functions of the SCMC in mammalian early embryogenesis.
HostingRepository	iProX
AnnounceDate	2024-09-03
AnnouncementXML	Submission_2024-09-03_00:23:35.108.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zihan Zhang
SpeciesList	scientific name: Spodoptera frugiperda; NCBI TaxID: 7108; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-03 00:20:31	ID requested
⏵ 1	2024-09-03 00:23:35	announced

Dataset with its publication pending

submitter keyword: 14-3-3 protein, SCMC, maternal effect gene.

Dong Deng
contact affiliation	Sichuan University
contact email	dengd@scu.edu.cn
lab head
Zihan Zhang
contact affiliation	Sichuan University
contact email	m18710996428@163.com
dataset submitter

iProX dataset URI