PXD055472-1

PXD055472 is an original dataset announced via ProteomeXchange.

Title	Saliva and salivary pellicle composition and proteomic profile in smokers vs. non-smokers and its effect on dental erosion (saliva data)
Description	Aim: This study aimed to analyse the salivary composition and proteomic profile of saliva and the salivary pellicle in smokers compared to non-smokers, and to examine potential differences of the erosion-protective capacity of the salivary pellicle. Materials and Methods: Twenty-five smokers and 25 non-smokers were included. Unstimulated and stimulated saliva samples were analysed regarding flow rate, pH, buffer capacity, calcium, phosphate, fluoride, and protein content. Salivary pellicles were formed intraorally on resin specimens (n=8). Saliva and salivary pellicle samples were used for data-independent acquisition mass spectrometry. Proteins were identified using the UniProtKB Homo sapiens reference proteome. For the saliva samples, proteomes of 15 commonly observed bacterial species from the oral microbiome were also included. In an in situ experiment, the participants used intraoral splints loaded with bovine enamel and dentin specimens for 120 min (each n=4). Pellicle-covered specimens were extraorally eroded (1mL HCl, pH 2.3, 60 s). Calcium release into the acid was determined photometrically and compared to pellicle-free specimens (control, each n=30). Differentially abundant proteins between smokers and non-smokers were identified using t tests (≥1.5-fold differences and padj.≤0.05). In addition, a pathway enrichment analysis of the associated genes was performed (KEGG, Reactome). Statistical analysis of the salivary samples was performed by one-way ANOVAs followed by pairwise t tests, t tests, Pearson correlations, and multiple linear regression analysis (p<0.05). Results: Except for phosphate in stimulated saliva (smokers: 5.5±2.1 mmol/L, non-smokers: 3.6±1.0 mmol/L; padj.=0.003), salivary parameters were not significantly different between smokers and non-smokers. For both saliva and the salivary pellicle, gene set enrichment analysis indicated common biological themes of genes enriched in either the smokers or the non-smokers. Despite the different proteomic profiles, the erosion-protective capacity of the salivary pellicle of smokers and non-smokers did not differ, and calcium release was not correlated with or predicted by the assessed salivary parameters. The salivary pellicle had a significant erosion-protective effect in enamel compared to the control (41.4±6.3 nmol/mm2), but no differences between smokers (33.2±10.6 nmol/mm2, padj.=0.001 ) and non-smokers (32.7±8.6 nmol/mm2, padj.=0.001) were found. Calcium release did not correlate with or was predicted by the assessed salivary parameters. Conclusion: The proteomic profiles of both unstimulated and stimulated saliva and the salivary pellicle differ between smokers and non-smokers. Evidence for different activity of different biological metabolic pathways such as carcinogesis exists. However, there was no detectable influence on the erosion-protective capacity of the pellicle.
HostingRepository	PRIDE
AnnounceDate	2026-07-01
AnnouncementXML	Submission_2026-06-30_17:20:12.363.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-02 04:18:42	ID requested
⏵ 1	2026-06-30 17:20:12	announced

10.1016/J.JDENT.2026.106517;

submitter keyword: dental erosion, salivary pellicle, smoker,Saliva, proteome

Christof Lenz
contact affiliation	Core Facility Proteomics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055472
     1. Label: PRIDE project
     2. Name: Saliva and salivary pellicle composition and proteomic profile in smokers vs. non-smokers and its effect on dental erosion (saliva data)