PXD055468-1

PXD055468 is an original dataset announced via ProteomeXchange.

Title	Signatures of Proteomics and Glycoproteomics Reveal Liraglutide Ameliorates NAFLD by Regulating Specific Metabolic Homeostasis in Mice
Description	Liraglutide, a GLP-1 receptor agonist approved for diabetes and obesity, shows promise in treating NAFLD, the most prevalent chronic liver disease globally. Despite its therapeutic potential, the systematic molecular regulations underlying Liraglutide effects on NAFLD remain underexplored, indicating the crucial need to fully understand its mechanism and facilitate its use in NAFLD treatment.Based on this,we established NAFLD modele by feeding C57/BL6 mice a high-fat diet (HFD). After administration of liraglutide injection to HFD mice, liver biochemical parameters were measured to assess the efficacy of liraglutide. Mouse livers were extracted for label-free quantitative proteomics and glycoproteomics analysis to summarize the mechanism of action of liraglutide in ameliorating NAFLD. Meanwhile, liraglutide hydrogels were prepared and characterized to extend the interval of liraglutide injection administration. Our research showed that liraglutide therapeutic effect on NAFLD was largely driven by significant glycosylation changes, which have a more profound impact than changes in overall protein expression. These insights into Liraglutide regulatory networks and targets offered valuable guidance for optimizing NAFLD treatment and advancing its clinical application.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:34:47.676.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dapeng Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	N-glycosylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-02 02:48:26	ID requested
⏵ 1	2025-12-14 16:34:48	announced

10.1016/j.jpha.2025.101273;

Chen Y, Liu C, Yang Q, Yang J, Zhang H, Zhang Y, Feng Y, Liu J, Li L, Li D, Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice. J Pharm Anal, 15(11):101273(2025) [pubmed]

submitter keyword: liraglutide, proteomics, fatty acid, N-glycosylation site, nonalcoholic fatty liver disease

Dapeng Li
contact affiliation	Key Laboratory of Drug Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
contact email	dpli@scu.edu.cn
lab head
Dapeng Li
contact affiliation	Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug, Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
contact email	dpli@scu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055468
     1. Label: PRIDE project
     2. Name: Signatures of Proteomics and Glycoproteomics Reveal Liraglutide Ameliorates NAFLD by Regulating Specific Metabolic Homeostasis in Mice