PXD055377-1

PXD055377 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of intra- and extracellular vesicles from cytotoxic CD8+ αβ T cells and γδ T cells
Description	Although belonging to different branches of the immune system, cytotoxic CD8+ αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and released to the immunological synapse upon target cell encounter. EVs of activated and expanded cytotoxic CD8+ αβ T cells or γδ T cells were enriched from culture supernatants by differential and ultracentrifugation and characterized by a comparative proteomic profiling with tandem mass tags (TMT10plex).
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:03:59.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-30 02:17:43	ID requested
⏵ 1	2025-05-07 02:04:00	announced

10.3390/cells13201745;

Griesel L, Kaleja P, Tholey A, Lettau M, Janssen O, T Cells. Cells, 13(20):(2024) [pubmed]

submitter keyword: exosomes, lysosome-related effector vesicles (LREVs), CD8+ T cells, αβ T cells, cytotoxic granules, cytotoxic effector proteins, γδ T cells,cytotoxic T cells, proteomic profiling, extracellular vesicles (EVs)

Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055377

PRIDE project URI

• PRIDE
  1. PXD055377
     1. Label: PRIDE project
     2. Name: Comparative analysis of intra- and extracellular vesicles from cytotoxic CD8+ αβ T cells and γδ T cells