PXD055367-1
PXD055367 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Laboratory evolution in Novosphingobium aromaticivorans enables rapid catabolism of a model lignin-derived aromatic dimer |
| Description | Lignin contains a variety of interunit linkages, which leads to a range of potential decomposition products that can be used as carbon and energy sources by microbes. B-O-4 linkages are the most common in native lignin and associated catabolic pathways have been well characterized. However, the fate of the mono-aromatic intermediates that result from B-O-4 dimer cleavage has not been fully elucidated. Here, we used experimental evolution to identify mutant strains of Novosphingobium aromaticivorans with improved catabolism of a model aromatic dimer containing a B-O-4 linkage, guaiacylglycerol-b-guaiacyl ether (GGE). We identified several parallel causal mutations, including a single nucleotide polymorphism in the promoter of an uncharacterized gene that roughly doubled the growth yield with GGE. We characterized the associated enzyme and demonstrated that it oxidizes an intermediate in GGE catabolism, B-hydroxypropiovanillone, to vanilloyl acetaldehyde. Identification of this enzyme and its key role in GGE catabolism furthers our understanding of catabolic pathways for lignin-derived aromatic compounds. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-12-20 |
| AnnouncementXML | Submission_2024-12-20_08:18:22.556.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dana Carper |
| SpeciesList | scientific name: Novosphingobium aromaticivorans; NCBI TaxID: 48935; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-29 15:30:27 | ID requested | |
| ⏵ 1 | 2024-12-20 08:18:22 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Novosphingobium aromaticivorans, Lignin, Laboratory evolution |
Contact List
| Richard J. Giannone | |
|---|---|
| contact affiliation | Oak Ridge National Laboratory |
| contact email | giannonerj@ornl.gov |
| lab head | |
| Dana Carper | |
| contact affiliation | Oak Ridge National Laboratory |
| contact email | carperdl@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095729/ |
to receive all new ProteomeXchange dataset release announcements!
