PXD055362 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Two Types of Axonal Muscarinic Acetylcholine Receptors Mediate Formation of Saliva Cocktail in the Tick Ixodes ricinus |
| Description | The long blood feeding duration of hard ticks relies on the continuous modulation of saliva composition to manipulate host haemostatic and immune systems. While the exogenous muscarinic acetylcholine receptor (mAChR) agonist, pilocarpine, is a potent inducer of tick salivation, the underlying control mechanisms of endogenous stimulation remain unclear. Here, we characterised two pharmacologically distinct types of mAChRs (A and B) in the genome of the Ixodes ricinus tick. A combination of molecular dynamics and directed mutagenesis revealed the atypical muscarinic profile of the type-B receptor. Immunolabelling followed by in vivo pharmacology and proteomic analyses suggested that the composition of secreted saliva is a result of a complex interplay between different central neurons and specific regions of the salivary gland (SG), mediated by distinct axonal mAChR types. This system likely functions upstream of a neuropeptide signalling cascade, ultimately controlling different salivation subunits in SG. Our study describes a unique model of regulation of tick SG activity in a medically relevant tick species. This knowledge holds broader implications for understanding tick-host interactions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-05 |
| AnnouncementXML | Submission_2026-02-05_05:26:24.027.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Filip Dyčka |
| SpeciesList | scientific name: Ixodes ricinus; NCBI TaxID: NEWT:34613; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-29 08:53:19 | ID requested | |
| ⏵ 1 | 2026-02-05 05:26:24 | announced | |
Publication List
Keyword List
| submitter keyword: synganglion,muscarinic receptors, neurosecretory cells, pharmacology, tick, axons, saliva, salivary gland acini |
Contact List
| Ladislav Šimo |
|---|
| contact affiliation | ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700 Maisons-Alfort, France |
| contact email | ladislav.simo@vet-alfort.fr |
| lab head | |
| Filip Dyčka |
|---|
| contact affiliation | University of South Bohemia in Ceske Budejovice
Faculty of Science
Branisovska 1760
Ceske Budejovice |
| contact email | dycka@mail.muni.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055362
- Label: PRIDE project
- Name: Two Types of Axonal Muscarinic Acetylcholine Receptors Mediate Formation of Saliva Cocktail in the Tick Ixodes ricinus
