PXD055357-1

PXD055357 is an original dataset announced via ProteomeXchange.

Title	Chromatin cell cycle proteomics reveals nuclear metabolism
Description	The progression of the cell cycle is regulated by the expression of specific genes and fluctuations in cellular metabolic states. By conducting cell cycle-based transcriptomics, proteomics, and metabolomics analyses, previous research has identified cell cycle-dependent alterations at the molecular, protein, and metabolic levels. However, the significance of protein compartmentalization, coupled with the evidence that metabolic enzymes can localize to the nucleus and influence chromatin states, suggests that fluctuations in nuclear metabolism may play a role in regulating cell cycle progression. In this study, we present a systematic approach to investigating the chromatin environment at a cell cycle resolution. By focusing on metabolic enzymes, we were able to ascertain that phosphatidylinositol metabolism localizes to the nucleus and undergoes alterations throughout the cell cycle. Moreover, the inhibition of phosphatidylinositol metabolism affects histone methylation in a manner that is dependent on the cell cycle, which ultimately impairs cell cycle progression. Our study demonstrates that nuclear metabolism is a dynamic process that shapes the chromatin environment and establishes a direct connection between nuclear metabolism and the cell cycle.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:41:23.700.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Savvas Kourtis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-29 08:13:04	ID requested
⏵ 1	2026-06-28 16:41:24	announced

10.1002/advs.202501083;

Ga, ñ, ez-Zapater A, Kourtis S, Elb, æ, k CR, Espinar L, Toro-M, á, rquez C, Coll-Manzano A, Smiriglia A, Garc, í, a-L, ó, pez L, Wiegand L, Guirola M, Fontaine F, Morandi A, M, ü, ller AC, Sdelci S, Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Adv Sci (Weinh), 13(34):e01083(2026) [pubmed]

submitter keyword: metabolism, cell cycle,chromatin, enzymes

Sara Sdelci
contact affiliation	Center for genomic regulation Barcelona
contact email	sara.sdelci@crg.eu
lab head
Savvas Kourtis
contact affiliation	CRG
contact email	kourtissavvas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD055357

PRIDE project URI

• PRIDE
  1. PXD055357
     1. Label: PRIDE project
     2. Name: Chromatin cell cycle proteomics reveals nuclear metabolism