PXD055299 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A CDK1 phospho-switch reprograms TRAIP to unload replisomes in mitosis |
| Description | Cells entering mitosis with incompletely replicated DNA face catastrophic chromosome segregation failure. During interphase, the replisome-associated E3 ubiquitin ligase TRAIP ubiquitylates barriers in front of the fork to allow replisome progression. In mitosis, TRAIP is reprogrammed from a trans-acting to a cis-acting ligase that can ubiquitylate the replisome itself. This enables the processing of unreplicated DNA by promoting replisome disassembly, fork breakage, and joining of the broken chromosome arms. Here, we describe a mechanism for this reprogramming: the ATPase TTF2 is recruited to the replisome, where its noncatalytic N-terminal domain tethers Cyclin B-CDK1-phosphorylated TRAIP to the leading strand DNA polymerase ε in a geometry that allows replisome ubiquitylation. Thus, a phospho-regulated architectural switch alters replisome organization in mitosis to safeguard genome integrity before chromosome segregation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-03 |
| AnnouncementXML | Submission_2026-07-03_07:32:00.770.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Markus Räschle |
| SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: NEWT:8355; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-28 09:19:25 | ID requested | |
| ⏵ 1 | 2026-07-03 07:32:01 | announced | |
Publication List
Keyword List
| submitter keyword: replication termination genomic stabilitym itosis |
Contact List
| Markus Räschle |
|---|
| contact affiliation | Molecular Genetics |
| contact email | raeschle@rhrk.uni-kl.de |
| lab head | |
| Markus Räschle |
|---|
| contact affiliation | Technical University Kaiserslautern |
| contact email | raeschle@rhrk.uni-kl.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD055299 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055299
- Label: PRIDE project
- Name: A CDK1 phospho-switch reprograms TRAIP to unload replisomes in mitosis
