PXD055289-1

PXD055289 is an original dataset announced via ProteomeXchange.

Title	Decoding pancreatic islet cell diversity through cell type-resolved proteomics and phosphoproteomics
Description	Islets of Langerhans are micro-organs scattered throughout the pancreas. They are composed of insulin-producing beta cells, glucagon-producing alpha cells, and somatostatin-producing delta cells. While their transcriptome has been extensively analyzed, protein-level information remains limited due to cell scarcity and purification challenges. Here, we combine cell sorting with highly sensitive mass spectrometry to create the first in depth proteomic atlas of pancreatic islet cells. We achieved a depth exceeding 6,000 proteins per endocrine cell population, discovering new cell type-enriched ones. Deep proteomics profiling upon interferon gamma (IFNγ) treatment, mimicking inflammation in type 1 diabetes demonstrated that all three endocrine cell types were inflamed upon IFNγ stimulation. Resolving the phosphoproteomic landscape of alpha, beta and delta cells with more than 7,000 unique phosphosites per cell type provided insights into cell-specific signaling. This multi-omics atlas offers a valuable resource for understanding pancreatic islet biology in health and disease.
HostingRepository	PRIDE
AnnounceDate	2025-10-02
AnnouncementXML	Submission_2025-10-02_09:49:59.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 04:47:29	ID requested
⏵ 1	2025-10-02 09:49:59	announced

Dataset with its publication pending

submitter keyword: mass spectrometry,phosphoproteomic, mouse

Matthias Mann
contact affiliation	Director Dept. Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 82152 Martinsried (near Munich) Germany
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD055289

PRIDE project URI

• PRIDE
  1. PXD055289
     1. Label: PRIDE project
     2. Name: Decoding pancreatic islet cell diversity through cell type-resolved proteomics and phosphoproteomics