PXD055284-1
PXD055284 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DSSBU: novel cross-linker tested on bovine serum albumin and human hemoglobin |
| Description | Protein cross-linking has assumed an irreplaceable role in structural proteomics. Recently, significant efforts have been made to develop novel MS-cleavable reagents. These cross-linkers enhance the reliability of cross-link identification. Presently, only water-insoluble MS-cleavable cross-linkers are commercially available. However, the comprehensive analysis of the various chemical and structural motifs inherent in proteins depends on the targeting of different protein sites with varying degrees of hydrophilicity. We introduce a new MS-cleavable cross-linker called disulfodisuccinimidyl dibutyric urea (DSSBU), which we have developed in-house. DSSBU contains an N-hydroxysulfosuccinimide (sulfo-NHS) reactive group. It therefore serves as a water-soluble counterpart to the commercially available and widely used disuccinimidyl dibutyric urea (DSBU). To investigate the applicability of DSSBU, we compared the efficacy of four similar cross-linkers—bis[sulfosuccinimidyl] suberate (BS3), disuccinimidyl suberate (DSS), DSBU and DSSBU on bovine serum albumin. We also compared efficacy of DSBU and DSSBU on human hemoglobin. Our results demonstrate that the superior water solubility of DSSBU enables to avoid the usage of polar solvents such as DMSO but still maintaining the effectivity of MS-cleavable crosslinker such as DSBU. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-05-07 |
| AnnouncementXML | Submission_2025-05-07_02:23:55.634.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Petra Junková |
| SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-28 03:28:25 | ID requested | |
| ⏵ 1 | 2025-05-07 02:23:56 | announced |
Publication List
| 10.1016/j.jprot.2024.105330; |
| Swati B, Jakub S, Ale, š M, Petra J, Martin H, cross-linker. J Proteomics, 310():105330(2025) [pubmed] |
Keyword List
| submitter keyword: DSSBU, hemoglobin, XL-MS, MS-cleavable cross-linker, BSA |
Contact List
| Josef Cvačka | |
|---|---|
| contact affiliation | Proteomics, Josef Cvačka Group, Institute of Organic Chemistry and Biochemistry of the CzechAcademy of Sciences; Flemingovo náměstí 542/2 160 00 Praha 6; Czech Republic |
| contact email | josef.cvacka@uochb.cas.cz |
| lab head | |
| Petra Junková | |
| contact affiliation | Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague |
| contact email | petra.junkova@uochb.cas.cz |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055284 |
| PRIDE project URI |
Repository Record List
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