PXD055279-1

PXD055279 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of a Toxoplasma gondii sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate
Description	The apicomplexan parasite Toxoplasma gondii has a complex life cycle involving multiple host species. Access to sexual stages and sporozoite-containing oocysts, essential for the parasite’s environmental transmission, is limited and requires animal experiments with cats. In vitro alternatives and resource-efficient methods are needed to improve our understanding of these crucial stages. Several molecular factors and transcriptional switches have been identified in recent years that are responsible for differentiation. Their genetic depletion or drug-induced inhibition, targeting the histone deacetylase HDAC3 in tachyzoites, leads to the expression of non-tachyzoite genes, including genes of sexual stages and the oocyst. This opens up the possibility of studying aspects of these stages in vitro. Here, we applied this concept and show that apicidin, a commercially available HDAC3 inhibitor, could be used to identify the hitherto unknown antigen of the sporozoite-specific monoclonal antibody G1/19 in tachyzoites. Using mass spectrometry of immunoprecipitated G1/19 target protein from apicidin-treated cultures, we identified it as SporoSAG. In addition, for the much less abundant sporozoite-specific protein LEA860, apicidin treatment was still sufficient to induce a detectable protein level in immunofluorescence microscopy. We discuss further use cases for apicidin-treated tachyzoites as surrogates for sexual stages and oocysts and address the limitations of this approach. Collectively, the paucity of material of sexual stages and oocysts from T. gondii can be overcome to some extent without the need for cat-derived material.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:02:21.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joerg Doellinger
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 02:01:25	ID requested
⏵ 1	2025-05-07 02:02:22	announced

10.1093/femsmc/xtae034;

Warschkau D, Klein S, Schadt E, Doellinger J, Schares G, Seeber F, sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate. FEMS Microbes, 6():xtae034(2025) [pubmed]

submitter keyword: Toxoplasma gondii, Protein-Protein Interaction, IP-MS

Peter Lasch
contact affiliation	ZBS6: Proteomics and Spectroscopy, Robert Koch-Institute
contact email	LaschP@rki.de
lab head
Joerg Doellinger
contact affiliation	Proteomics and Spectroscopy (ZBS6), Robert Koch-Institute
contact email	Doellingerj@rki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055279

PRIDE project URI

• PRIDE
  1. PXD055279
     1. Label: PRIDE project
     2. Name: Proteomic identification of a Toxoplasma gondii sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate