PXD055278-1

PXD055278 is an original dataset announced via ProteomeXchange.

Title	Nitric Oxide Regulates Stomatal Development and Stress Responses by S-Nitrosylation-Mediated Inhibiting Phosphorylation of MPK6
Description	In plants, stomata on the aerial epidermis play critical roles in various biological processes, including gas exchange, photosynthesis, transpiration, and immunity. Stomatal development is negatively and positively regulated by the mitogen-activated protein kinase (MAPK) cascade and nitric oxide (NO), respectively. However, the regulatory scheme of stomatal development by these signaling pathways remains elusive. Here, we show that NO-modulated stomatal development is genetically dependent on MPK3 and MPK6. Moreover, NO-mediated S-nitrosylation of MPK6 at Cys-201 inhibits its phosphorylation, resulting in the stabilization of SPCH, a master regulator of stomatal lineage initiation, thereby promoting stomatal development. Remarkably, a MPK6C201S mutation confers NO insensitivity during stomatal development and stress responses. We propose that NO positively regulates stomatal development and stress responses by the S-nitrosylation-mediated inhibition of the MPK6 activity, thus identifying a mechanism linking the coupled NO-MAPK signaling to specific biological outputs.
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-20_17:45:14.089.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongyan Guo
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 01:45:43	ID requested
⏵ 1	2025-02-20 17:45:14	announced

Dataset with its publication pending

submitter keyword: Stomata

nitric oxide

S-nitrosylation

MAPK

MPK6

SPCH

stress responses

ABA

Jianru Zuo
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Science
contact email	jrzuo@genetics.ac.cn
lab head
Hongyan Guo
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	hyguo@genetics.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055278
     1. Label: PRIDE project
     2. Name: Nitric Oxide Regulates Stomatal Development and Stress Responses by S-Nitrosylation-Mediated Inhibiting Phosphorylation of MPK6