PXD055277 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | NovoBoard: a comprehensive framework for evaluating the false discovery rate and accuracy of de novo peptide sequencing |
Description | De novo peptide sequencing is a fundamental research area in mass spectrometry (MS) based proteomics. However, those methods have often been evaluated using a couple of simple metrics that do not fully reflect their overall performance. Moreover, there has not been an established method to estimate the false discovery rate (FDR) and the significance of de novo peptide-spectrum matches (PSMs). Here we propose NovoBoard, a comprehensive framework to evaluate the performance of de novo peptide sequencing methods. The framework consists of diverse benchmark datasets (including tryptic, nontryptic, immunopeptidomics, and different species), and a standard set of accuracy metrics to evaluate the fragment ions, amino acids, and peptides of the de novo results. More importantly, a new approach is designed to evaluate de novo peptide sequencing methods on target-decoy spectra and to estimate their FDRs. Our results thoroughly reveal the strengths and weaknesses of different de novo peptide sequencing methods, and how their performances depend on specific applications and the types of data. Our FDR estimation also shows that some tools may perform better than the others in distinguishing between de novo PSMs and random matches, and can be used to assess the significance of de novo PSMs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-28 |
AnnouncementXML | Submission_2024-08-28_02:00:59.386.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ngoc Hieu Tran |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-28 01:42:18 | ID requested | |
⏵ 1 | 2024-08-28 02:00:59 | announced | |
2 | 2024-10-22 06:56:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: false discovery rate,de novo peptide sequencing |
Contact List
Baozhen Shan |
contact affiliation | Bioinformatics Solutions Inc. |
contact email | bshan@bioinfor.com |
lab head | |
Ngoc Hieu Tran |
contact affiliation | Bioinformatics Solutions Inc. |
contact email | htran@bioinfor.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055277
- Label: PRIDE project
- Name: NovoBoard: a comprehensive framework for evaluating the false discovery rate and accuracy of de novo peptide sequencing