PXD055273-1

PXD055273 is an original dataset announced via ProteomeXchange.

Title	Surviving Martian Brines: Proteomic Analysis of Escherichia coli’s Adaptation to Perchlorate Stress
Description	Brines, potentially formed by the deliquescence and freezing point depression of highly hygroscopic salts, such as perchlorates (ClO4-), may allow for the spatial and temporal stability of liquid water on present-day Mars. It is therefore of great interest to explore the microbial habitability of Martian brines, for which our current understanding is, however, still limited. Putative microbes growing in the perchlorate-rich Martian regolith may be harmed due to the induction of various stressors including osmotic, chaotropic, and oxidative stress. We adapted the model organism Escherichia coli to increasing sodium perchlorate concentrations and used a proteomic approach to identify the adaptive phenotype. Separately, the microbe was adapted to elevated concentrations of NaCl and glycerol, which enabled us to distinguish perchlorate-specific adaptation mechanisms from those in response to osmotic, ion and water activity stress. We found that the perchlorate-specific stress-response focused on pathways alleviating damage to nucleic acids, presumably caused by increased chaotropic and/ or oxidative stress. The significant enrichments that have been found include DNA repair, RNA methylation and de novo IMP biosynthesis. Our study provides insights into the adaptive mechanisms necessary for microorganisms to survive under perchlorate stress, with implications for understanding the habitability of Martian brines.
HostingRepository	PRIDE
AnnounceDate	2025-01-21
AnnouncementXML	Submission_2025-01-21_04:16:50.552.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joerg Doellinger
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-27 23:46:27	ID requested
⏵ 1	2025-01-21 04:16:50	announced

Dataset with its publication pending

submitter keyword: Mars, adaptation,Astrobiology, brines, perchlorate, habitability

Peter Lasch
contact affiliation	ZBS6: Proteomics and Spectroscopy, Robert Koch-Institute
contact email	LaschP@rki.de
lab head
Joerg Doellinger
contact affiliation	Proteomics and Spectroscopy (ZBS6), Robert Koch-Institute
contact email	Doellingerj@rki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055273
     1. Label: PRIDE project
     2. Name: Surviving Martian Brines: Proteomic Analysis of Escherichia coli’s Adaptation to Perchlorate Stress