⮝ Full datasets listing
PXD055269-1
PXD055269 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | O-SaMPle: an optimized workflow for human salivary metaproteomic analysis reveals taxonomic and functional dysbiosis in IBD patients |
| Description | We developed an Optimized Salivary MetaProteomic sample analysis workflow (O-SaMPle) to enrich salivary bacteria and reduce host-derived interferences for in-depth analysis of the oral metaproteome. This workflow integrates using selective microbial enrichment buffer and differential centrifugation. In details, the selective microbial enrichment buffer can dissolves host proteins and detaches microorganisms from cells while preserving the integrity of the microbes by using a combination of low concentrations of denaturing agents and detergents, followed differential centrifugation can effectively isolate and enrich oral microbiota from human saliva or mouth rinse samples. Furthermore, we applied O-SaMPle to analyzing mouth rinse samples from IBD patients, and revealed dysregulated microbes and their corresponding dysregulated pathways under disease conditions. |
| HostingRepository | iProX |
| AnnounceDate | 2024-08-26 |
| AnnouncementXML | Submission_2025-12-04_23:34:55.247.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jinhui Yuan |
| SpeciesList | scientific name: human oral bacteria ensemble; NCBI TaxID: 150596; |
| ModificationList | S-carboxamidomethyl-L-cysteine; acetylated residue; methionine oxidation with neutral loss of 64 Da |
| Instrument | Orbitrap Fusion; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-27 19:01:44 | ID requested | |
| ⏵ 1 | 2025-12-04 23:34:56 | announced |
Publication List
| Yuan J, Sun B, Li M, Yang C, Zhang L, Chen N, Chen F, Li L, OSaMPle workflow for salivary metaproteomics analysis reveals dysbiosis in inflammatory bowel disease patients. NPJ Biofilms Microbiomes, 11(1):63(2025) [pubmed] |
Keyword List
| submitter keyword: human oral metaproteome |
Contact List
| Leyuan Li | |
|---|---|
| contact affiliation | State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics |
| contact email | lileyuan@ncpsb.org.cn |
| lab head | |
| Jinhui Yuan | |
| contact affiliation | State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics |
| contact email | yuanjinh2021@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
