PXD055228 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | GPC3-mediated metabolic rewiring of diabetic mesenchymal stromal cells enhances their cardioprotective functions via pyruvate kinase M2 activation |
Description | Mesenchymal stromal cells (MSC) are promising stem cell therapy for treating cardiovascular and other degenerative diseases. Diabetes affects the functional capability of MSC and impedes cell-based therapy. Despite numerous studies, the impact of diabetes on MSC myocardial reparative activity, metabolic fingerprint, and the mechanism of dysfunction remains inadequately understood. We demonstrated that the transplantation of diabetic-MSC (db/db-MSC) into the ischemic myocardium of mice does not confer cardiac benefit post-MI. Metabolomic studies identified defective energy metabolism in db/db-MSC. Furthermore, we found that glypican-3 (GPC3), a heparan sulfate proteoglycan, is highly upregulated in db/db-MSC and is involved in metabolic alterations in db/db-MSC via pyruvate kinase M2 activation. GPC3-knockdown reprogrammed-db/db-MSC restored their energy metabolic rates, immunomodulation, angiogenesis, and cardiac reparative activities. Together, these data indicate that GPC3-metabolic reprogramming in diabetic MSC may represent a strategy to enhance MSC based therapeutics for myocardial repair in diabetic patients |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-04 |
AnnouncementXML | Submission_2024-09-04_08:21:20.987.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055228 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Manveen Sethi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-27 03:05:04 | ID requested | |
⏵ 1 | 2024-09-04 08:21:21 | announced | |
2 | 2024-10-22 06:57:41 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Metabolomics, Immunomodulation,Mesenchymal stromal cells, Heart failure, Glypican |
Contact List
Raj Kishore |
contact affiliation | Aging and Cardiovascular Discovery Center, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA |
contact email | raj.kishore@temple.edu |
lab head | |
Manveen Sethi |
contact affiliation | Boston University |
contact email | msethi@bu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055228
- Label: PRIDE project
- Name: GPC3-mediated metabolic rewiring of diabetic mesenchymal stromal cells enhances their cardioprotective functions via pyruvate kinase M2 activation