PXD055204-1

PXD055204 is an original dataset announced via ProteomeXchange.

Title	Fecal metaproteomics of ketogenic diet - case study
Description	Metaproteomics is a valuable approach to characterize the biological functions involved in the gut microbiota (GM) response to dietary interventions. Ketogenic diets (KDs) are very effective in controlling seizure severity and frequency in drug-resistant epilepsy (DRE) and in the weight loss management in obese/overweight individuals. This case study provides proof of concept for the suitability of metaproteomics to monitor changes in taxonomic and functional GM features in an individual on a short-term very low-calorie ketogenic diet (VLCKD, 4 weeks), followed by a low-calorie diet (LCD). A marked increase in Akkermansia and Pseudomonadota was observed during VLCKD and reversed after the partial reintroduction of carbohydrates (LCD), in agreement with the results of previous metagenomic studies. In functional terms, the relative increase in Akkermansia was associated with an increased production of proteins involved in response to stress and biosynthesis of gamma-aminobutyric acid. In addition, VLCKD caused a relative increase in enzymes involved in the synthesis of the beta-ketoacid acetoacetate and of the ketogenic amino acid leucine. Our data support the potential of fecal metaproteomics to investigate the GM-dependent effect of KD as a therapeutic option in obese/overweight individuals and DRE patients.
HostingRepository	PRIDE
AnnounceDate	2024-12-23
AnnouncementXML	Submission_2024-12-23_02:32:42.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Tanca
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-26 07:26:21	ID requested
⏵ 1	2024-12-23 02:32:42	announced

10.1002/PMIC.202400191;

submitter keyword: ketogenic diet,Akkermansia, gut microbiota, overweight, GABA, glutamate decarboxylase, metaproteomics

Sergio Uzzau
contact affiliation	Department of Biomedical Sciences, University of Sassari, Sassari, Italy
contact email	uzzau@uniss.it
lab head
Alessandro Tanca
contact affiliation	Department of Biomedical Sciences, University of Sassari, Sassari, Italy
contact email	aletanca@uniss.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD055204

PRIDE project URI

• PRIDE
  1. PXD055204
     1. Label: PRIDE project
     2. Name: Fecal metaproteomics of ketogenic diet - case study