PXD055201-1

PXD055201 is an original dataset announced via ProteomeXchange.

Title	APEX2 of BAX, BAK and DRP1 in healthy and apoptotic cells
Description	To identify components of mitochondrial apoptotic foci, Flag-APEX2-tagged constructs targeting Bax, Bak, and Drp1 were generated. These constructs are designed to localize to foci at the mitochondrial outer membrane (MOM) during apoptosis. Following biotinylation with BP and H2O2, quantitative mass spectrometry was used to identify neighbouring proteins. Notably, the mitochondrial outer membrane (MOM) contains porins that allow the free diffusion of molecules, potentially resulting in the biotinylation of intermembrane space (IMS) and mitochondrial inner membrane (MIM)-resident proteins. To comprehensively analyse the protein composition of apoptotic foci, three independent quantitative mass spectrometry experiments were conducted. Each experiment involved comparing untreated (control) cells with apoptotic cells induced by staurosporine treatment. By utilizing APEX2-tagged constructs in live cells, the subcellular localization of proteins under both healthy and apoptotic conditions was preserved. Moreover, consistent biotinylation patterns were observed across all three constructs, validating the reliability of the approach for studying dynamic protein complexes in apoptosis.
HostingRepository	PRIDE
AnnounceDate	2026-03-27
AnnouncementXML	Submission_2026-03-27_05:12:18.425.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-26 06:09:27	ID requested
⏵ 1	2026-03-27 05:12:18	announced

Dataset with its publication pending

submitter keyword: BAK, APEX2, BAX,Apoptotic foci, DRP1

Ana J. Garcia-Saez
contact affiliation	Institute for Genetics, University of Cologne, Cologne, Germany
contact email	ana.garcia@uni-koeln.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD055201

PRIDE project URI

• PRIDE
  1. PXD055201
     1. Label: PRIDE project
     2. Name: APEX2 of BAX, BAK and DRP1 in healthy and apoptotic cells