PXD055190-1

PXD055190 is an original dataset announced via ProteomeXchange.

Title	Sustained non-photochemical quenching and photoprotection of PSII repair cycle during combined high light and low temperature stress in lettuce
Description	In nature, light and other environmental conditions are constantly changing, requiring plants to have several overlapping regulatory mechanisms to keep light reactions and metabolism in balance. Here, we show that high light (HL) induces a much stronger down-regulation of light reactions when lettuce plants are exposed to 1500 µmol photons m−2 s−1 for 4 h at 13°C (low temperature, LT) compared to 23°C (growth temperature, GT). GT/HL treatment induced non-photochemical quenching (NPQ), which relaxed during 1 h recovery in darkness. In contrast, LT/HL treatment induced an exceptionally high NPQ that only partially relaxed during 1 h in darkness at GT. Such a high sustained NPQ (sNPQ) cannot be explained by the canonical NPQ mechanism(s). Instead, sNPQ was associated with partial disassembly of PSII LHCII complexes and a transient increase in phosphorylation of the minor antenna protein LHCB4. This coincided with increased expression of the light-harvesting like proteins SEP2 and ELIP1.2, the PSII assembly proteins HCF173 and LPA3, and some enzymes of pigment metabolism. These results lead us to propose that LHCB4 phosphorylation-dependent disassembly of PSII-LHCII super-complexes allows SEP2 to bind to CP47 and hypothetically quenches the inner PSII core antenna, while free CP43 released during PSII repair is proposed to be protected by LPA3.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:42:58.244.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dorota Muth-Pawlak
SpeciesList	scientific name: Lactuca sativa; NCBI TaxID: NEWT:4236;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-26 01:52:44	ID requested
⏵ 1	2026-02-08 16:43:01	announced

10.1111/ppl.70298;

Lempi, ä, inen T, Muth-Pawlak D, Vainonen JP, Rintam, ä, ki E, Tikkanen M, Aro EM, Moderate Temperature Reduction Changes the High-Light Acclimation Strategy of Lettuce Plants. Physiol Plant, 177(3):e70298(2025) [pubmed]

submitter keyword: thylakoid proteome, photoprotection, photosynthesis, photoinhibition,lettuce

Eva-Mari Aro
contact affiliation	Molecular Plant Biology Department of Technology University of Turku Finland
contact email	evaaro@utu.fi
lab head
Dorota Muth-Pawlak
contact affiliation	University of Turku
contact email	dokrmu@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055190
     1. Label: PRIDE project
     2. Name: Sustained non-photochemical quenching and photoprotection of PSII repair cycle during combined high light and low temperature stress in lettuce