PXD055171 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparison of lung tissue-derived extracellular vesicles using multiple dissociation methods for profiling protein biomarkers |
| Description | Extracellular vesicles (EVs) operate as chemical messengers that facilitate intercellular communication. Emerging evidence has demonstrated that lung tissue-derived EVs play pivotal roles in pulmonary physiological processes and have potential as biomarkers and therapeutics for lung diseases. Multiple methods have been proposed for the isolation of lung tissue-derived EVs. However, the effects of different tissue pre-treatments on lung EV isolation and subsequent disease biomarker discovery have not yet been comprehensively investigated. In this study, we compared the physical characteristics, recovery yields, and protein compositions of EVs isolated from lung tissues using three methods based on different tissue dissociation principles. Methodologically, the beneficial roles of blood perfusion and gentle meshing were emphasized based on their impact on EV yield and purity. These results demonstrate that different methods enrich distinct subpopulations of EVs that exhibit significant differences in their protein cargo and surface properties. These disparities directly affect the diagnostic detection of marker proteins related to lung diseases, including lung tumors, asthma, and pulmonary fibrosis. Collectively, these findings highlight the variations in EV characteristics resulting from the applied approaches, and offer compelling suggestions for guiding researchers in selecting a suitable isolation method based on downstream functional studies and clinical applications. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_06:59:30.905.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | YUE HAN |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-24 09:42:15 | ID requested | |
| 1 | 2024-09-23 18:52:42 | announced | |
| ⏵ 2 | 2024-10-22 06:59:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: disease biomarkers, proteomics,lung EVs, tissue dissociation methods |
Contact List
| Yue Han |
|---|
| contact affiliation | School of Engineering Medicine, Beihang University, Beijing, China |
| contact email | by1910013@buaa.edu.cn |
| lab head | |
| YUE HAN |
|---|
| contact affiliation | Beihang University |
| contact email | 814157218@qq.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055171
- Label: PRIDE project
- Name: Comparison of lung tissue-derived extracellular vesicles using multiple dissociation methods for profiling protein biomarkers
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