PXD055147-1

PXD055147 is an original dataset announced via ProteomeXchange.

Title	Interrogating the interaction of fission yeast RNA-binding protein Puf3 with the Ccr4-Not complex using crosslinking mass spectrometry
Description	mRNA decay is a key determinant of gene regulation, initiated by the shortening of mRNA poly(A) tails by the CCR4-NOT deadenylation complex. Specificity is achieved through RNA adaptors—RNA-binding proteins that recruit CCR4-NOT to specific substrate mRNAs in a regulated manner. However, the molecular mechanisms underlying this specificity remain unclear in many cases. In this study, we applied crosslinking MS to investigate the interaction between the fission yeast RNA-binding protein Puf3 and the Ccr4-Not complex.
HostingRepository	PRIDE
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_04:16:10.373.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055147
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhuo Chen
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-23 11:57:02	ID requested
⏵ 1	2025-09-03 04:16:10	announced

10.6019/PXD055147;

submitter keyword: Crosslinking MS, IDR, protein-protein interaction, RNA binding protein Puf3, Ccr4-Not complex

Juri Rappsilber
contact affiliation	Technische Universität Berlin, Chair of Bioanalytics, Germany. Wellcome Centre for Cell Biology, University of Edinburgh, UK. Si-M/"Der Simulierte Mensch", a Science Framework of Technische Universität Berlin and Charité - Universitätsmedizin Berlin, Germany.
contact email	juri.rappsilber@tu-berlin.de
lab head
Zhuo Chen
contact affiliation	Technischen Universität Berlin
contact email	zhuo.chen@tu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055147
     1. Label: PRIDE project
     2. Name: Interrogating the interaction of fission yeast RNA-binding protein Puf3 with the Ccr4-Not complex using crosslinking mass spectrometry