PXD055123-1

PXD055123 is an original dataset announced via ProteomeXchange.

Title	Global proteomics indicates subcellular-specific anti-ferroptotic responses to ionizing radiation
Description	Cells have many protective mechanisms against background levels of ionising radiation (IR) orchestrated by molecular changes in expression, post-translation modifications and subcellular localisation. Radiotherapeutic treatment in oncology attempts to overwhelm such mechanisms, but radio-resistance is an ongoing challenge. Here, global subcellular proteomics combined with Bayesian modelling identified 544 differentially localised proteins in A549 cells upon 6 Gy x-ray exposure, revealing subcellular-specific changes of proteins involved in ferroptosis, an iron-dependent cell death, suggestive of potential radio-resistance mechanisms. These observations were independent of expression changes, emphasizing the utility of global subcellular proteomics and the promising prospect of ferroptosis-inducing therapies for combatting radioresistance.
HostingRepository	PRIDE
AnnounceDate	2024-12-16
AnnouncementXML	Submission_2024-12-16_14:29:59.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Josie Christopher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-23 04:03:22	ID requested
⏵ 1	2024-12-16 14:30:00	announced

10.1016/J.MCPRO.2024.100888;

submitter keyword: LC-MS/MS, irradiation, Human, subcellular fractionation, A549 cells

Kathryn S. Lilley
contact affiliation	Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, UK.
contact email	k.s.lilley@bioc.cam.ac.uk
lab head
Josie Christopher
contact affiliation	University of Cambridge
contact email	josiechristopher93@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD055123

PRIDE project URI

• PRIDE
  1. PXD055123
     1. Label: PRIDE project
     2. Name: Global proteomics indicates subcellular-specific anti-ferroptotic responses to ionizing radiation