PXD055117-1

PXD055117 is an original dataset announced via ProteomeXchange.

Title	Proximity-based labeling of MuSK interactome
Description	Muscle specific kinase (MuSK) is a receptor tyrosine kinase that plays a crucial role in development and maintenance of the neuromuscular junction (NMJ). Lack of MuSK is lethal and defective MuSK signaling causes muscle weakness. MuSK activity is tightly regulated and requires the nerve-derived heparansulphate proteoglycan Agrin. However, signal transduction induced by MuSK activation is not well understood. Here, we used proximity labeling to decipher the MuSK interactome by identifying and characterizing interaction partners of MuSK upon Agrin stimulation. To uncover the early and late events in the MuSK signaling cascade, muscle cells were stimulated with Agrin for 2 h, 4 h and 8 h. Overall, our study reveals that CrkL functions as a signaling hub to recruit different molecules for distinct signaling outcomes and contributes to a better understanding of the complex mechanisms that regulate NMJ formation.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_09:23:05.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-23 02:27:42	ID requested
⏵ 1	2026-08-17 09:23:06	announced

Dataset with its publication pending

submitter keyword: MuSK, TurboID, neuromuscular junction, proximity labeling, signaling

Ruth Herbst
contact affiliation	Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.
contact email	ruth.herbst@meduniwien.ac.at
lab head
Markus Hartl
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact email	proteomics@maxperutzlabs.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD055117

PRIDE project URI

• PRIDE
  1. PXD055117
     1. Label: PRIDE project
     2. Name: Proximity-based labeling of MuSK interactome