PXD055103-1

PXD055103 is an original dataset announced via ProteomeXchange.

Title	Meta-Analysis and DIA-MS-based Proteomic Investigation of COPD Patients and Asymptomatic Smokers in the Indian population
Description	Chronic obstructive pulmonary disease (COPD) is the second leading cause of death in India, primarily attributed to smoking. Asymptomatic smokers can develop COPD due to a combination of genetic, environmental, and molecular factors, highlighting the importance of early detection. Data-independent acquisition mass spectrometry (DIA-MS) proteomics provides an unbiased approach to analyzing proteomic profiles. This study is the first to utilize DIA-based proteomics to examine individual serum samples from three distinct male groups: healthy individuals (n=10), asymptomatic smokers (n=10), and COPD patients (n=10). This thorough analysis identified 667 proteins with a 1% false discovery rate. Differential expression analysis revealed 40 proteins distinguishing normal from asymptomatic smokers, 88 proteins differentiating COPD from healthy individuals, and 40 proteins differentiating COPD from asymptomatic smokers. Notably, proteins such as PRDX6, ELANE, PRKCSH, PRTN3, and MNDA may help distinguish COPD from asymptomatic smokers, while ELANE, H3-3A, IGHE, SLC4A1, and SERPINA11 could differentiate COPD from healthy subjects. Pathway enrichment and protein-protein interaction analyses highlighted significant changes in hemostasis, immune system functions, fibrin clot formation, and post-translational protein modifications. Key proteins were validated through a parallel reaction monitoring assay. Our findings identify critical protein markers in COPD patients, asymptomatic smokers, and healthy individuals, providing insights for clinicians to better understand the disease’s pathobiology, enhance disease management, and improve patient quality of life
HostingRepository	PanoramaPublic
AnnounceDate	2024-11-13
AnnouncementXML	Submission_2024-11-13_13:14:38.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gautam Sharma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 21:21:40	ID requested
⏵ 1	2024-11-13 13:14:39	announced

Sharma G, Gupta DP, Ganguly K, Anand MP, Srivastava S, Meta-Analysis and DIA-MS-Based Proteomic Investigation of COPD Patients and Asymptomatic Smokers in the Indian Population. J Proteome Res, 23(11):4973-4987(2024) [pubmed]

submitter keyword: COPD, smokers, asymptomatic smokers, healthy subjects, DIA, proteomics, Indian population

Sanjeeva Srivastava
contact affiliation	Professor
contact email	sanjeeva@iitb.ac.in
lab head
Gautam Sharma
contact affiliation	Indian Institute of Technology Bombay
contact email	graviksharma45@gmail.com
dataset submitter

Panorama Public dataset URI