PXD055096-1

PXD055096 is an original dataset announced via ProteomeXchange.

Title	Ultra-Photosensitization of Fluorescent Protein Chromophores towards Photocatalytic Proximity Labeling and Proteomic Profiling
Description	Due to relatively lower reactive oxygen species (ROS) quantum yield, photosensitizing fluorescent proteins (FP) (e.g. KillerRed) have been shown not suitable for photo-catalytic protein proximity labeling and downstream proteomic profiling applications. To acquire such functions, we engineer FP chromophores in a 12×12 combinatorial matrix of synthetic analogues, yielding maximally over 1000-fold enhancement of reactive oxygen species (ROS) production. We show the ROS yield is correlated to the directionality of “push-pull” effect of FP chromophores. After conjugation to HaloTag, the ultra-photosensitized FP mimics (namely upsFP) herein realizes photo-catalytic protein proximity labeling using nucleophilic amino substrates. A set of photochemical characterizations, theoretical calculation, and tandem mass spectrometry collectively reveals radical mediated labeling with expanded reactivity towards protein residues via type I photosensitization pathway. Finally, we showcase a proteomic profiling application by using the upsFP tag to resolve the dynamic interactome variations upon TAR DNA-binding protein 43 (TDP43) phase separation and suborganellar translocation. Together, this work demonstrates 3 orders of magnitude ultra- photosensitization enables photocatalytic proximity labeling and profiling functions for biomimetic fluorescent proteins.
HostingRepository	iProX
AnnounceDate	2024-08-23
AnnouncementXML	Submission_2024-08-22_20:16:07.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nan Zhao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 20:15:35	ID requested
⏵ 1	2024-08-22 20:16:07	announced

Dataset with its publication pending

submitter keyword: fluorescent protein, proximity labeling, photosensitization, proteomics, phase separation

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics
contact email	lihuazhang@dicp.ac.cn
lab head
Nan Zhao
contact affiliation	Dalian Institute of Chemical Physics
contact email	zhaonan@dicp.ac.cn
dataset submitter

iProX dataset URI