PXD055091-1

PXD055091 is an original dataset announced via ProteomeXchange.

Title	Structural basis of the mitochondrial RNA editing cascade in trypanosomes
Description	The discovery of RNA editing in trypanosomes introduced the concept of guide RNA (gRNA). To understand how the RECC1 and RECC2 complexes mediate gRNA-directed uridine deletions and insertions, respectively, in mitochondrial mRNAs, we determined their structures and RNA interactions. The RECC cores, consisting of distinct heterotetramers of active and inactive RNase III endonucleases, recognize gRNA–mRNA duplexes. Auxiliary zinc-finger domains distinguish deletion from insertion editing sites, while asymmetrical catalytic centers cleave the mRNA. Three peripheral heterotetramers composed of oligonucleotide-binding (OB-fold) proteins flexibly tether exonuclease, TUTase, and ligase enzymes to the cores, facilitating trimming or extension of cleaved mRNA and strand repair. An architectural tRNA stabilizes the RNase III–OB-fold interface. These findings illuminate the organization of the enzymatic cascade and suggest a mechanism for editing site recognition.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-15_15:34:16.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clinton Yu
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 14:52:26	ID requested
⏵ 1	2026-06-15 15:34:16	announced

Dataset with its publication pending

submitter keyword: RNA editing catalytic complex,trypanosoma, mitochondria, RNA editing, guide RNA editosome

Ruslan Afasizhev
contact affiliation	Boston University Goldman School of Dental Medicine Boston MA, 02118, USA
contact email	ruslana@bu.edu
lab head
Clinton Yu
contact affiliation	University of California, Irvine
contact email	clinton.yu@uci.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055091
     1. Label: PRIDE project
     2. Name: Structural basis of the mitochondrial RNA editing cascade in trypanosomes