PXD055090-2

PXD055090 is an original dataset announced via ProteomeXchange.

Title	Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment
Description	Epilepsy is characterized by hypersynchronous neuronal discharges, which are associated with an increased cerebral metabolic rate of oxygen and ATP demand. Uncontrolled seizure activity (status epilepticus) results in mitochondrial exhaustion and ATP depletion, which potentially generate energy mismatch and neuronal loss. Many cells can adapt to increased energy demand by increasing metabolic capacities. However, acute metabolic adaptation during epileptic activity and its relationship to chronic epilepsy remains poorly understood. We elicited seizure-like events (SLEs) in an in vitro model of status epilepticus for eight hours. Electrophysiological recording and tissue oxygen partial pressure recordings were performed. After eight hours of ongoing SLEs, we used proteomics-based kinetic modeling to evaluate changes in metabolic capacities. We compared our findings regarding acute metabolic adaptation to published proteomic and transcriptomic data from chronic epilepsy patients. Epileptic tissue acutely responded to uninterrupted SLEs by upregulating ATP production capacity. This was achieved by a coordinated increase in the abundance of proteins from the respiratory chain and oxidative phosphorylation system. In contrast, chronic epileptic tissue shows a 25-40% decrease in ATP production capacity. In summary, our study reveals that epilepsy leads to dynamic metabolic changes. Acute epileptic activity boosts ATP production, while chronic epilepsy reduces it significantly.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:58:08.521.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Loroch
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 14:03:58	ID requested
1	2024-09-12 07:53:27	announced
⏵ 2	2024-10-22 06:58:08	announced	2024-10-22: Updated project metadata.

10.3390/IJMS25179640;

submitter keyword: epilepsy

metabolic adaptation

status epilepticus

ATP production

shotgun proteomics

Stefan Loroch
contact affiliation	[1] QC-MS / Fa. Dr. Loroch, BioMedizinZentrum, Otto-Hahn-Straße 15, 44149 Dortmund [2] Medizinisches Proteom-Center, Center for Protein Diagnostics (PRODI), Medical Faculty, Ruhr-University Bochum, Bochum, Germany
contact email	stefan@loroch.email
lab head
Stefan Loroch
contact affiliation	Medizinisches Proteome-Center der Ruhr Universität Bochum
contact email	stefan@loroch.email
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD055090

PRIDE project URI

• PRIDE
  1. PXD055090
     1. Label: PRIDE project
     2. Name: Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment