PXD055087-1

PXD055087 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic profiling in Saccharomyces cerevisiae with abrogated binding of Nab3 to PIC2
Description	Whole-proteome profiling enabled us to compare proteomic differences between a Saccharomyces cerevisiae parental strain (PIC2-GFP) and two derived mutants lacking Nab3 RNA-binding sites in PIC2. Previous experiments had established that disrupting Nab3 binding to PIC2 while maintaining Nrd1 binding to the same transcript led to severe growth defects, larger cell size, delayed cell cycle and resistance to oxidative stress. Following characterization of a PIC2-overexpressing mutant we established that these phenotypes were not related to Pic2 overabundance. Therefore, the purpose of this experiment was to determine (i) whether the proteomic signature of the mutant lacking Nab3 RNA-binding sites in PIC2 could account for the observed phenotypes and (ii) if the proteins that were differentially expressed in such mutant were targeted by Nab3 and Nrd1.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-11-30_16:47:02.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sofia Esteban Serna
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 12:49:00	ID requested
⏵ 1	2025-11-30 16:47:03	announced

Publication List

10.1093/nar/gkaf1199;
Esteban-Serna S, Wid, é, n T, Gwynne M, Farquhar I, Duchen MR, Swain PS, Granneman S, A transcription termination mechanism for maintaining homogeneous protein expression. Nucleic Acids Res, 53(21):(2025) [pubmed]

10.1093/nar/gkaf1199;

Esteban-Serna S, Wid, é, n T, Gwynne M, Farquhar I, Duchen MR, Swain PS, Granneman S, A transcription termination mechanism for maintaining homogeneous protein expression. Nucleic Acids Res, 53(21):(2025) [pubmed]

Keyword List

submitter keyword: gene expression noise suppression, transcriptional co-regulation, energy homeostasis, cellular fitness.,Nrd1-Nab3-Sen1, mRNA attenuation, cell cycle, transcription termination, cell size

submitter keyword: gene expression noise suppression, transcriptional co-regulation, energy homeostasis, cellular fitness.,Nrd1-Nab3-Sen1, mRNA attenuation, cell cycle, transcription termination, cell size

Contact List

Sander Granneman
contact affiliation	Centre for Engineering Biology, School of Biological Sciences, University of Edinburgh, United Kingdom
contact email	sander.granneman@ed.ac.uk
lab head
Sofia Esteban Serna
contact affiliation	Centre for Engineering Biology, University of Edinburgh
contact email	s.esteban-serna@ed.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD055087
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD055087

PRIDE project URI

Repository Record List

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