PXD055070-1

PXD055070 is an original dataset announced via ProteomeXchange.

Title	SpLICe (Spectral Library of Immune Cells).
Description	Spectral libraries fulfill multiple functions in biological and analytical applications. For biologists, these libraries provide a valuable resource to verify the presence and abundance of specific proteins or pathways within a selected cell type, and thus determine the feasibility of further experiments. Despite advances, existing libraries are incomplete and provide researchers only a limited amount of information. To address this, we introduce the Spectral Library of Immune Cells (SpLICe), a comprehensive resource covering five main cell populations comprising nearly 9,000 protein groups, 123,554 proteotypic peptides, and 135,240 spectra. SpLICe supports the quantification of more than half of total murine proteins annotated by UniProtKB/Swiss-Prot, enabling monitoring of selected proteins or pathways from Reactome pathways, GO Biological Process, GO Molecular Function, or GO Cellular Component databases. The platform also provides insights about the relative abundance of proteins and their associated transcripts and supports the generation of targeted mass spectrometry assays by identifying available proteotypic peptides and evaluating their suitability for targeted MS analyses via an integrated scoring system.
HostingRepository	PRIDE
AnnounceDate	2025-11-21
AnnouncementXML	Submission_2025-11-21_05:30:54.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannah Voss
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 06:13:41	ID requested
⏵ 1	2025-11-21 05:30:55	announced

10.1038/S41597-025-04829-9;

submitter keyword: BMDCs., Bioinformatics,Proteomics, Spectral library, CD8 T-cells, Mouse, Immunology, B-cells, CD4 T-cells, BMDMs

Dr. Olga Shevchuk
contact affiliation	Group of Immunoproteomics, Department of Immunodynamics, Institute for Experimental Immunology and Imaging, Medical Research Centre, University Hospital Essen Hufelandstraße 55 D-45147 Essen, Germany
contact email	Olga.Shevchuk@uk-essen.de
lab head
Hannah Voss
contact affiliation	Medical Research Centre University Hospital Essen Hufelandstraße 55 D-45147 Essen, Germany
contact email	hannahluise.voss@uk-essen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD055070

PRIDE project URI

• PRIDE
  1. PXD055070
     1. Label: PRIDE project
     2. Name: SpLICe (Spectral Library of Immune Cells).