PXD055052-1

PXD055052 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of adult CNS from Rhoa cKOs and CTRs mice
Description	Oligodendrocyte differentiation and subsequent myelination rely on strict regulatory mechanisms and molecules. Here we aimed at understanding the role of the Rho GTPase RhoA during oligodendrocyte differentiation and myelination in vivo. Using a conditional cell-specific ablation of Rhoa (Rhoa fl/fl mice crossed with Cnp-Cre mice) in oligodendrocytes, we show that RhoA controls the timing and progression differentiation and myelination. To identify putative mechanisms underlying the Rhoa cKO phenotype, we performed quantitative label-free LC-MS/MS proteomics on optic nerves from Rhoa cKO (Rhoa fl/fl Cre+) and control mice (Rhoa fl/fl Cre-) at 30 days post-birth (P30). Manual annotation of the differentially abundant proteins showed a large modulation of several proteins clusters associated with actin cytoskeleton, signal transduction and mitochondria.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_05:42:07.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joana Paes de Faria
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 06:07:06	ID requested
⏵ 1	2024-11-05 05:42:07	announced

10.1002/GLIA.24640;

submitter keyword: RhoA, Optic Nerve,Oligodendrocyte, Rho GTPase, Mouse, LC-MS/MS

João B. Relvas
contact affiliation	Instituto de Investigação e Inovação em Saúde (i3S) - Universidade do Porto
contact email	jrelvas@i3s.up.pt
lab head
Joana Paes de Faria
contact affiliation	Instituto de Investigação e Inovação em Saúde (i3S) - Universidade do Porto
contact email	joana.paesdefaria@i3s.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD055052

PRIDE project URI

• PRIDE
  1. PXD055052
     1. Label: PRIDE project
     2. Name: Proteomic analysis of adult CNS from Rhoa cKOs and CTRs mice