⮝ Full datasets listing
PXD055022-1
PXD055022 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CX3CR1 modulates migration of resident microglia towards brain injury |
| Description | Microglia are the innate immune cells of the brain. They are highly motile cells that screen the surrounding brain parenchyma on a minute timescale. In an ATP-dependent manner, microglia extend their processes towards an injury in the central nervous system (CNS). Microglia migrate towards ischemic sites within hours in vivo. However, whether the fractalkine receptor (CX3CR1) influences microglial migration remains unknown. Label free proteomic profiling pinpointed elevated motor dysfunction and differential changes in Rho GTPase signaling activity that indicated a dysregulated actin cytoskeleton signaling in Cx3cr1-deficient cortex. To investigate migration properties of microglia, we carried out 4-hour interval two-photon in vivo imaging for 72 hours after a laser lesion in the cortex. Cx3cr1-deficient mice showed enhanced migration towards the lesion. Not only cell body migration, but also length and velocity of microglial fine processes extending towards the lesion were increased in Cx3cr1-deficient microglia. Migration and number of migrating microglia remained unchanged in Ccr2-deficient mice, indicating that monocyte-derived macrophages/microglia did not contribute to migration and proliferation around the injury. These results demonstrate microglia migration towards CNS injury and suggest a role for CX3CR1 in modulating this process. Manipulating microglia migration may become an important target for the treatment of CNS-injury. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_08:48:03.864.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rabah Soliymani |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Oxidation; Deamidated |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-20 06:05:20 | ID requested | |
| ⏵ 1 | 2026-08-25 08:48:04 | announced |
Publication List
| Wagner J, Antony H, Hoyer C, Lundgr, é, n K, Soliymani R, Crux S, Justus L, Keppler K, Steffen J, Kurts C, Engel DR, Herms J, Ł, a, ł, owski M, Fuhrmann M, CX3CR1 Modulates Migration of Resident Microglia Towards Cortical Laser-Induced Lesions. Glia, 74(10):e70207(2026) [pubmed] |
Keyword List
| submitter keyword: microglia, CX3CR1 fractalkine, microglia migration, CNS-injury, imaging |
Contact List
| Maciej Lalowski | |
|---|---|
| contact affiliation | Helsinki Institute for Life Science (HiLIFE) and Faculty of Medicine, Biochemistry/Developmental Biology, Meilahti Clinical Proteomics Core Facility, University of Helsinki, Helsinki, FI-00014 |
| contact email | maciej.lalowski@helsinki.fi |
| lab head | |
| Rabah Soliymani | |
| contact affiliation | University of Helsinki |
| contact email | rabah.soliymani@helsinki.fi |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095652/ |
