PXD055018-1
PXD055018 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | PRMT1-mediated methylation regulates MLL2 stability and gene expression |
Description | The interplay between multiple transcription factors precisely regulates eukaryotic transcription. Here, we report that the protein methyltransferases, MLL2 and PRMT1, interact directly and act collectively to regulate gene expression. PRMT1 binds to the N-terminal region of MLL2, considered an intrinsically disordered region, and methylates multiple arginine residues within its RGG/RG motifs. Notably, overexpression of PRMT1 decreased poly-ubiquitylation of MLL2, whereas mutations on methylation sites in MLL2 increased MLL2 poly-ubiquitylation, suggesting that PRMT1-mediated methylation stabilizes MLL2. MLL2 and PRMT1 cooperatively stimulated the expression of a chromosomal reporter gene in a PRMT1-mediated, MLL2-methylation–dependent manner. RNA-seq analysis found that MLL2 and PRMT1 jointly regulate the expression of genes involved in cell membrane and extracellular matrix functions, and depletion of either resulted in impaired cell migration and invasion. Our study provides evidence that PRMT1-mediated MLL2 methylation regulates MLL2 protein stability and the expression of their target genes. |
HostingRepository | jPOST |
AnnounceDate | 2024-10-21 |
AnnouncementXML | Submission_2024-10-20_18:12:35.324.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jihyun Kim |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-08-20 00:31:43 | ID requested | |
⏵ 1 | 2024-10-20 18:12:35 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: MLL2, PRMT1 |
Contact List
Jaehoon Kim | |
---|---|
lab head | |
Jihyun Kim | |
contact affiliation | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
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