PXD054952-1

PXD054952 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins interacting with the small GTPase Arl4D by liposome flotation assay and mass spectrometry
Description	N-terminal myristoylation serves as an important modification for membrane insertion and concrete membrane anchoring of the small GTPases ADP-ribosylation factor (Arf) and ADP ribosylation factor-like (Arl). To simulate the binding topology of Arl4D on the membrane, we performed the liposome flotation assay in which N-terminally His-tagged Arl4D is loaded onto Ni-NTA-incorporated liposomes and the C-terminus of Arl4D is accessible to its interacting proteins. Liposomes containing Arl4D (active form Arl4D Q80L and inactive form Arl4D T35N) and Arl4D-interacting proteins derived from HeLa cell lysates were identified by a sucrose gradient in combination with mass spectrometry analysis. Specifically, we identified mitogen-activated protein kinase 1 (Erk2) as a specific interacting protein of Arl4D Q80L. We further demonstrate a novel role for Arl4D whereby the active form of Arl4D recruits Erk1/2 to the plasma membrane and promotes Pak1 T212 phosphorylation to promote cell migration.
HostingRepository	PRIDE
AnnounceDate	2025-05-10
AnnouncementXML	Submission_2025-05-10_00:41:01.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Jung Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-16 02:13:49	ID requested
⏵ 1	2025-05-10 00:41:02	announced

Dataset with its publication pending

submitter keyword: Erk2,small GTPase Arl4D, liposome flotation

Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@gmail.com
lab head
Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054952
     1. Label: PRIDE project
     2. Name: Identification of proteins interacting with the small GTPase Arl4D by liposome flotation assay and mass spectrometry