PXD054916-1

PXD054916 is an original dataset announced via ProteomeXchange.

Title	Kidney transcriptomic and proteomic analyses provide new insight into the pathogenesis of IgA nephropathy in mice
Description	Background:Immunoglobulin A nephropathy (IgAN) is a common clinically refractory disease, and its pathogenesis remains unclear. Studying the pathogenesis of IgA nephropathy is of great significance for the clinical treatment of IgA nephropathy. Methods:The IgAN mouse model was established through oral administration of bovine serum albumin, subcutaneous injection of carbon tetrachloride, and tail vein injection of lipopolysaccharide. Transcriptome sequencing and TMT protein sequencing were employed to analyze the mRNA and protein expression profiles in the kidney tissue of IgAN mice. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were conducted on differentially expressed genes (DEGs) and proteins (DEPs). A protein-protein interaction (PPI) network diagram was constructed for overexpressed genes and proteins, allowing for the identification of hub DEGs and DEPs. Additionally, the correlation between core genes and proteins and the glomerular filtration rate was examined using the Nephroseq v5 database. Results:The serum biochemical indicators and renal tissue pathology tests confirmed the successful construction of the IgAN mouse model. A total of 415 differentially expressed genes (DEGs) were identified through transcriptome sequencing, while 99 differentially expressed proteins (DEPs) were obtained via TMT protein sequencing. The intersection of these datasets yielded 21 co-expressed DEGs/DEPs. Functional enrichment analysis revealed significant enrichment in signaling pathways related to inflammatory response, immune regulation, amino acid metabolism, and lipid metabolism. Additionally, LCN2, HMGCR, and HDC were identified as the key hub DEGs/DEPs within the protein-protein interaction (PPI) network. Clinical correlation analysis demonstrated a clear relationship between LCN2, HMGCR, HDC, and glomerular filtration rate. Conclusions:This study offers new insights into the pathogenesis of IgAN and presents novel targets for its diagnosis and treatment.
HostingRepository	iProX
AnnounceDate	2024-08-15
AnnouncementXML	Submission_2025-01-01_20:00:19.374.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xingxing Zhuang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	TMT6plex reporter fragment
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-15 02:42:58	ID requested
⏵ 1	2025-01-01 20:00:19	announced

Dataset with its publication pending

submitter keyword: IgA nephropathy, Transcriptomic, Proteomic

Jiarong Gao
contact affiliation	Department of Pharmacy, Chaohu Hospital of Anhui Medical University
contact email	zyfygjr2006@ahtcm.edu.cn
lab head
Xingxing Zhuang
contact affiliation	Department of Pharmacy, Chaohu Hospital of Anhui Medical University
contact email	zxx900913@126.com
dataset submitter

iProX dataset URI