PXD054869-1

PXD054869 is an original dataset announced via ProteomeXchange.

Title	Myeloid lineage C3 induces reactive gliosis and neuronal stress during CNS inflammation
Description	Complement component C3 mediates pathology in several CNS neurodegenerative diseases, but the cellular and molecular mechanisms leading to neuronal injury remain unclear. We examined how C3 deletion affects glial profiles and anterior visual pathway pathology in an animal model of neuroinflammation, EAE. scRNA-seq from mouse brain and optic nerve revealed that C3 expression defined disease-associated glial subtypes characterized by increased expression of genes associated with mTOR activation, cell metabolism, and translation. This was confirmed with proteomic analysis of the optic nerves of C3KO and WT EAE mice. Deletion of C3 restored glia towards homeostatic profiles. Myeloid-derived C3 mediated injury in optic nerve axons and retinal ganglion cells (RGCs) at disease peak. Our study supports a direct role for C3 in activating the mTOR-ribosomal biogenesis axis in glia which subsequently mediate early neuro-axonal stress and synapse loss.
HostingRepository	PRIDE
AnnounceDate	2025-02-18
AnnouncementXML	Submission_2025-02-17_16:05:07.068.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chanhyun na
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-14 02:43:57	ID requested
⏵ 1	2025-02-17 16:05:07	announced

Dataset with its publication pending

submitter keyword: neuroinflammation, neuronal injury, EAE, multiple sclerosis,Complement component 3, microglia, optic neuritis, retina, OCT

Chan-Hyun Na
contact affiliation	Associate Professor of Neurology Institute for Cell Engineering Johns Hopkins University School of Medicine
contact email	chanhyun@jhmi.edu
lab head
chanhyun na
contact affiliation	Johns Hopkins University
contact email	chanhyun.na@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054869
     1. Label: PRIDE project
     2. Name: Myeloid lineage C3 induces reactive gliosis and neuronal stress during CNS inflammation