PXD054824-1

PXD054824 is an original dataset announced via ProteomeXchange.

Title	Rapid microwave fixation of the brain reveals seasonal changes in the phosphoproteome of hibernating thirteen-lined ground squirrels
Description	Hibernating mammals such as the thirteen-lined ground squirrel (Ictidomys tridecemlineatus) experience significant reductions in oxidative metabolism and body temperature when entering a state known as torpor. Animals entering or exiting torpor do not experience permanent loss of brain function or other injuries, and the processes that enable such neuroprotection are not well understood. To gain insight into changes in protein function that occur in dramatically different physiological states, we performed quantitative phosphoproteomics experiments on thirteen-lined ground squirrels that are summer active, winter torpid, and spring active. An important aspect of our approach was the use of focused microwave irradiation of the brain to sacrifice the animals and rapidly inactivate phosphatases and kinases to preserve the native phosphoproteome. Overall, our results showed pronounced changes in phosphorylated proteins for the transitions into and out of torpor, including for proteins involved in gene expression and DNA repair, cellular plasticity, and human disease. In contrast, the transition between active states showed minimal changes. This study offers valuable insight into the global changes of brain phosphorylation in hibernating mammals, the results of which may be relevant to the future therapeutic strategies for brain injury.
HostingRepository	PRIDE
AnnounceDate	2025-01-23
AnnouncementXML	Submission_2025-01-23_10:31:38.664.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Spermophilus tridecemlineatus (Thirteen-lined ground squirrel); NCBI TaxID: 43179;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 12:34:39	ID requested
⏵ 1	2025-01-23 10:31:39	announced

10.1021/acschemneuro.4c00635;

Abid MSR, Naldrett MJ, Alvarez S, Eichhorn CD, Andrews MT, Checco JW, Rapid Microwave Fixation of the Brain Reveals Seasonal Changes in the Phosphoproteome of Hibernating Thirteen-Lined Ground Squirrels. ACS Chem Neurosci, 16(3):428-438(2025) [pubmed]

submitter keyword: hibernation

brain

phosphoproteomics

TMT labeling

focused microwave

gene expression

neuroprotection

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD054824

PRIDE project URI

• PRIDE
  1. PXD054824
     1. Label: PRIDE project
     2. Name: Rapid microwave fixation of the brain reveals seasonal changes in the phosphoproteome of hibernating thirteen-lined ground squirrels