PXD054815-1

PXD054815 is an original dataset announced via ProteomeXchange.

Title	Regulatory Coordination of Antisense RNA and Encoded Protein in Cell Cycle.
Description	Antisense RNAs constitute a significant part of the human genome, yet their functions in key physiological processes remain poorly understood due to complexity and the lack of suitable models. Here, we report the intricate regulatory co-ordination between antisense RNA and its encoded protein involved in safeguarding the essential physiological processes during interphase and mitosis, using antisense RNA ARHGEF17-AS1 and its encoded protein, MSEP as a model system. Mechanistically, ARHGEF17-AS1 orchestrates actin-cytoskeleton organization in interphase and mitotic spindle pole integrity via the RNF10-RPS3-Twinfilin-Dynein pathway, involving monoubiquitination dependent functional switch of RPS3 during interphase to mitosis. While MSEP ensures spindle integrity through the TPX2-Aurora A pathway. Thus, our investigations provide molecular insights into how ARHGEF17-AS1 and its protein product MSEP maintain critical cell cycle stage-specific processes through unique and coordinated actions. These findings establish a compelling model for investigating the collaborative actions between antisense RNA and its encoded protein in cellular physiology.
HostingRepository	PRIDE
AnnounceDate	2026-08-28
AnnouncementXML	Submission_2026-08-28_00:19:42.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liisa Arike
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	methylthiolated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 05:53:43	ID requested
⏵ 1	2026-08-28 00:19:42	announced

Dataset with its publication pending

submitter keyword: Aurora-A., cell division, Open reading frame (ORF),Antisense RNA, Mitotic spindle essential protein (MSEP), TPX2

Chandrasekhar Kanduri
contact affiliation	Professor in Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Sweden,
contact email	kanduri.chandrasekhar@gu.se
lab head
Liisa Arike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD054815

PRIDE project URI

• PRIDE
  1. PXD054815
     1. Label: PRIDE project
     2. Name: Regulatory Coordination of Antisense RNA and Encoded Protein in Cell Cycle.