PXD054796-1
PXD054796 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Spatiotemporally-resolved mapping of extracellular proteomes via in vivo-compatible TyroID |
| Description | Extracellular proteins play pivotal roles in both intracellular signaling and intercellular communications in health and disease. While recent advancements in proximity labeling (PL) methods, such as peroxidase- and photocatalyst-based approaches, have facilitated the resolution of extracellular proteomes, their in vivo compatibility remains limited. Here, we report TyroID, an in vivo-compatible PL method for unbiased mapping of extracellular proteins with high spatiotemporal resolution. TyroID employs plant- and bacteria-derived tyrosinases to produce reactive o-quinone intermediates, enabling the labeling of multiple residues on endogenous proteins with bioorthogonal handles, thereby allowing for their identification via chemical proteomics. We validate TyroID's specificity by mapping extracellular proteomes and HER2-neighboring proteins using nanobody-directed recombinant tyrosinases. Demonstrating its superiority over other PL methods, TyroID enables in vivo mapping of extracellular proteomes, including mapping HER2-proximal proteins in tumor xenografts, quantifying the turnover of plasma proteins and labeling hippocampal-specific proteomes in live mouse brains. TyroID emerges as a potent tool for investigating protein localization and molecular interactions within living organisms. |
| HostingRepository | iProX |
| AnnounceDate | 2024-11-21 |
| AnnouncementXML | Submission_2025-01-21_18:23:48.307.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yankun Wang |
| SpeciesList | scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-11 20:22:07 | ID requested | |
| ⏵ 1 | 2025-01-21 18:23:48 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: proximity labeling |
Contact List
| Wei Qin | |
|---|---|
| contact affiliation | Tsinghua University |
| contact email | weiqin@tsinghua.edu.cn |
| lab head | |
| Yankun Wang | |
| contact affiliation | Tsinghua University |
| contact email | yankun_wang@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
to receive all new ProteomeXchange dataset release announcements!
