PXD054760-1

PXD054760 is an original dataset announced via ProteomeXchange.

Title	The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation
Description	The functions of integrins are tightly regulated via multiple mechanisms including trafficking and degradation. Integrins are repeatedly internalized, routed into the endosomal system and either degraded by the lysosome or recycled back to the plasma membrane. The ubiquitin system dictates whether internalized proteins are degraded or recycled. Here, we used a genetic screen and proximity-dependent biotin identification to identify deubiquitinase(s) that control integrin surface levels. We found that a ternary deubiquitinating complex, comprised of USP12 (or the homologous USP46), WDR48 and WDR20, stabilizes β1 integrin (Itgb1) by preventing ESCRT-mediated lysosomal degradation. Mechanistically, the USP12/46-WDR48-WDR20 complex removes ubiquitin from the cytoplasmic tail of internalized Itgb1 in early endosomes, which in turn prevents ESCRT-mediated sorting and Itgb1 degradation.
HostingRepository	PRIDE
AnnounceDate	2024-09-06
AnnouncementXML	Submission_2024-09-06_00:50:33.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-09 07:56:00	ID requested
⏵ 1	2024-09-06 00:50:34	announced
2	2024-10-22 06:57:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ubiquitination, USP12/USP46, ESCRT, integrin, DUB

Reinhard Fässler
contact affiliation	Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany
contact email	faessler@biochem.mpg.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD054760

PRIDE project URI

• PRIDE
  1. PXD054760
     1. Label: PRIDE project
     2. Name: The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation