PXD054750 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of DDX6 ubiquitylated lysines in cAMP-stimulated cells. stimulation |
| Description | DDX6 is an RNA helicase mostly located in the P-bodies, membrane-less organelles that play a fundamental role in the post-transcriptional regulation of gene expression. We found that stimulation of the G protein-coupled receptor (GPCR)-cAMP pathway induces a non-proteolytic polyubiquitylation of DDX6 mediated by the RING E3 ligase Praja2. To identify potential lysine residues of DDX6 accepting ubiquitin moieties mediated by Praja2, we performed a proteomic analysis on DDX6 purified from total lysates from cells treated with Forskolin, a diterpene that raises cAMP levels. The analysis identified K26 and K286 as ubiquitylated sites upon cAMP stimulation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-05-26 |
| AnnouncementXML | Submission_2025-05-25_16:11:15.211.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Luc Camoin |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-09 04:14:16 | ID requested | |
| ⏵ 1 | 2025-05-25 16:11:16 | announced | |
Publication List
Keyword List
| submitter keyword: DIA, polyubiquitin, P-bodies,cAMP, DDX6, LC-MS/MS |
Contact List
| Luc Camoin |
|---|
| contact affiliation | Marseille Protéomique, CRCM, Inserm, CNRS, Institut Paoli Calmettes, Aix-Marseille Université |
| contact email | luc.camoin@inserm.fr |
| lab head | |
| Luc Camoin |
|---|
| contact affiliation | Life Sciences |
| contact email | luc.camoin@inserm.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054750
- Label: PRIDE project
- Name: Identification of DDX6 ubiquitylated lysines in cAMP-stimulated cells. stimulation
