PXD054750-1

PXD054750 is an original dataset announced via ProteomeXchange.

Title	Identification of DDX6 ubiquitylated lysines in cAMP-stimulated cells. stimulation
Description	DDX6 is an RNA helicase mostly located in the P-bodies, membrane-less organelles that play a fundamental role in the post-transcriptional regulation of gene expression. We found that stimulation of the G protein-coupled receptor (GPCR)-cAMP pathway induces a non-proteolytic polyubiquitylation of DDX6 mediated by the RING E3 ligase Praja2. To identify potential lysine residues of DDX6 accepting ubiquitin moieties mediated by Praja2, we performed a proteomic analysis on DDX6 purified from total lysates from cells treated with Forskolin, a diterpene that raises cAMP levels. The analysis identified K26 and K286 as ubiquitylated sites upon cAMP stimulation.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:11:15.211.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-09 04:14:16	ID requested
⏵ 1	2025-05-25 16:11:16	announced

10.1038/s44319-025-00425-5;

Senatore E, Avolio R, Rinaldi L, Chiuso F, Oliva MA, D'Ambrosio C, Bianco AG, Dalla E, Pagnotta SM, Flammia R, Ambrosino C, Memoli D, Turacchio G, Mimoune SI, Toiron Y, Audebert S, Camoin L, Lignitto L, Scaloni A, Arcella A, Feliciello A, Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6. EMBO Rep, 26(9):2347-2377(2025) [pubmed]

submitter keyword: DIA, polyubiquitin, P-bodies,cAMP, DDX6, LC-MS/MS

Luc Camoin
contact affiliation	Marseille Protéomique, CRCM, Inserm, CNRS, Institut Paoli Calmettes, Aix-Marseille Université
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054750

PRIDE project URI

• PRIDE
  1. PXD054750
     1. Label: PRIDE project
     2. Name: Identification of DDX6 ubiquitylated lysines in cAMP-stimulated cells. stimulation