PXD054741-1

PXD054741 is an original dataset announced via ProteomeXchange.

Title	Time-course SILAC proteomics to determine the response to WEE1i in RPE-1 WT vs EIF2A-KO RPE-1 cells
Description	AZD1775 is an ATP-competitive inhibitor of WEE1 protein kinase. Inhibition of WEE1 causes hyperactivation of CDK1 and CDK2, which leads to premature mitosis and DNA replication stress. AZD1775 has been evaluated in patients with relapsed high grade serous ovarian cancer (HGSOC) and has shown promising results. However, not all HGSOC tumors responded to AZD1775 treatment, which calls for further investigation into the mechanisms of AZD1775 sensitivity. Based on a viral mutagenesis genome-scale screen in HAP1 cells to identify gene mutations that confer sensitivity to AZD1775 treatment, we showed that inactivation of the alternative translation initiation factor EIF2A confers sensitivity to AZD1775 in several cell line models, including RPE1 cells. Here we measured the differential response of RPE-1 WT or EIF2A-KO cells to WEE1i, at 4 different incubation times.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_07:19:47.176.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rolf de Boer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 23:14:20	ID requested
⏵ 1	2025-10-07 07:19:47	announced

Dataset with its publication pending

submitter keyword: human ovarian WEE1 EIF2A HGSOC

Marcel van Vugt
contact affiliation	Department of Medical Oncology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
contact email	m.vugt@umcg.nl
lab head
Rolf de Boer
contact affiliation	University Medical Center Groningen
contact email	h.r.de.boer@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD054741

PRIDE project URI

• PRIDE
  1. PXD054741
     1. Label: PRIDE project
     2. Name: Time-course SILAC proteomics to determine the response to WEE1i in RPE-1 WT vs EIF2A-KO RPE-1 cells